Micellar electrokinetic chromatography assay for the simultaneous quantification of urinary transferrin and albumin

被引：1

作者：

Jun-Feng Liu

Yi-Wei Wu

Yan-Hui Cheng

Xing-Wang Zhou

Hai-Li Zhang

De-Yan Han

机构：

[1] Hubei Normal University,Department of Chemistry and Environmental Engineering and Hubei Key Laboratory of Pollutant Analysis & Reuse Technology

[2] Department of Clinical Laboratory,undefined

[3] the Second Hospital of Huangshi,undefined

来源：

Microchimica Acta | 2009年 / 165卷

关键词：

Transferrin; Albumin; Micellar electrokinetic chromatography; Radioimmunoassay; Human urine;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A micellar electrokinetic chromatography (MEKC) method for simultaneous determination of urinary transferrin and albumin has been developed. The performance of the assay was evaluated. Under the optimum conditions, the linearity of transferrin and albumin is in the range of 10~1000 mg L−1. The detection limits are 1.3 mg L−1 for transferrin and 1.1 mg L−1 for albumin, with relative standard deviations of less than 5.4%. The within-run and between-run coefficients of variation were <5.5% for transferrin and <8.2% for albumin, respectively. The recoveries of transferrin and albumin are 97.7~106.1%. The performance of the MEKC assay was compared with radioimmunoassay, and correlated well with this.

引用

页码：285 / 290

页数：5

共 105 条

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