Production of mannitol using Leuconostoc mesenteroides NRRL B-1149

被引:15
作者
Kim C.Y. [1 ]
Lee J.H. [2 ]
Kim B.H. [3 ]
Yoo S.K. [5 ]
Seo E.S. [1 ]
Cho K.S. [4 ]
Day D.F. [6 ]
Kim D. [1 ,2 ]
机构
[1] Department of Material, Chemical and Biochemical Engineering, Chonnam National University
[2] Faculty of Applied Chemical Engineering, Chonnam National University
[3] Engineering Research Institute, Chonnam National University
[4] Department of Molecular Biotechnology, Chonnam National University
[5] Department of Food and Biotechnology, Joong Bu University, Geumsan-Gun
[6] Audubon Sugar Institute, Louisiana State University, Baton Rouge
关键词
Fed-batch fermentation; Fructose; Leuconostoc mesenteroides; Mannitol;
D O I
10.1007/BF02932977
中图分类号
学科分类号
摘要
A process for the production of mannitol from fructose (5% to 25%) using Leuconostoc mesenteroides NRRL B-1149 was investigated. Fermentations were carried out in batch or fed-batch fermentations without aeration at 28°C, pH 5.0. When 5% fructose was used in batch culture fermentation, the yield of mannitol was 78% of that expected theoretically. When the fructose concentration was increased to 10%, the yield dropped to 59.6% of the theoretical value. However, in the fed-batch culture, using 10% fructose, the yield was 81.9% of the theoretical value. In a 15% fructose fed-batch culture, with 5% fructose being added initially and the other 10% fructose being added as a continuous supply, the final yield was 83.7% of the theoretical yield. When 20% fructose was used in the same manner, the yield was 89.5% of theoretical yield.
引用
收藏
页码:234 / 236
页数:2
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