PCR-based specific detection of Ralstonia solanacearum race 4 strains

被引：40

作者：

Horita M. ^{[1
]}

Yano K. ^{[2
]}

Tsuchiya K. ^{[3
]}

机构：

[1] Natl. Agric. Res. Ctr. Hokkaido Reg., Toyohira-ku, Sapporo 062-8555

[2] Kochi Agricultural Research Center, Kochi

[3] Natl. Inst. of Agro-Environ. Sci., Ibaraki

来源：

Journal of General Plant Pathology | 2004年 / 70卷 / 5期

关键词：

Bacterial wilt; PCR detection; Ralstonia solanacearum; rep-PCR; Zingiberaceae crop;

D O I：

10.1007/s10327-004-0126-7

中图分类号：

学科分类号：

摘要：

Two primer sets were designed based on the sequence of polymorphic bands that were derived from repetitive sequence-based polymerase chain reaction (repPCR) fingerprinting and specifically detected in Ralstonia solanacearum race 4 strains (ginger, mioga, and curcuma isolates). One primer set (AKIF-AKIR) amplified a single band (165 bp) from genomic DNA obtained from all mioga and curcuma and some ginger isolates; another set (21F-21R) amplified one band (125bp) from the other ginger isolates. These primer sets did not amplify the bands from genomic DNA of other R. solanacearum strains or of other related bacteria. PCR detection limit for the pathogen was 2 × 102cfu.

引用

页码：278 / 283

页数：5

共 20 条

[1]

Buddenhagen I., Kelman A., Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum, Annu Rev Phytopathol, 2, pp. 203-230, (1964)

[2]

Elphinestone J.G., Hennessy J., Wilson J.K., Stead D.E., Sensitivity of different methods for the detection of Ralstonia solanacearum in potato tuber extracts, EPPO Bull, 26, pp. 663-678, (1996)

[3]

Hayward A., Moffett M., Pegg K., Bacterial wilt of ginger in Queensland, Queensland J Agric Anim Sci, 24, pp. 1-5, (1967)

[4]

Hayward A.C., Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum, Annu Rev Phytopathol, 29, pp. 65-87, (1991)

[5]

He L., Sequeira L., Kelman A., Characteristics of strains of Pseudomonas solanacearum from China, Plant Dis, 67, pp. 1357-1361, (1983)

[6]

Hendrick C.A., Sequeira L., Lipopolysaccharide-defective mutants of the wilt pathogen Pseudomonas solanacearum, Appl Environ Microbiol, 48, pp. 94-101, (1984)

[7]

Horita M., Tsuchiya K., Phenotypic characteristics and cluster analysis of Japanese and reference strains of Ralstonia solanacearum, Ann Phytopathol Soc Jpn, 65, pp. 604-611, (1999)

[8]

Horita M., Tsuchiya K., Genetic diversity of Japanese strains of Ralstonia solanacearum, Phytopathology, 91, pp. 399-407, (2001)

[9]

Kelman A., The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance on a tetrazolium medium, Phytopathology, 44, pp. 693-695, (1954)

[10]

Louws F.J., Fulbright D.W., Stephens C.T., De Brujin F.J., Specific genomic fingerprint of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR, Appl Environ Microbiol, 60, pp. 2286-2295, (1994)

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