Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

被引：43

作者：

Asano M. ^{[1
,2
]}

Kubota S. ^{[1
]}

Nakanishi T. ^{[1
]}

Nishida T. ^{[1
]}

Yamaai T. ^{[3
]}

Yosimichi G. ^{[1
]}

Ohyama K. ^{[1
]}

Sugimoto T. ^{[3
]}

Murayama Y. ^{[2
]}

Takigawa M. ^{[1
]}

机构：

[1] Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama

[2] Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama

[3] Department of Oral Functional Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama

来源：

Cell Communication and Signaling | / 3卷 / 1期

关键词：

Collagen Synthesis; Connective Tissue Growth Factor; Periodontal Ligament; Periodontal Tissue; Tooth Germ;

D O I：

10.1186/1478-811X-3-11

中图分类号：

学科分类号：

摘要：

Background: CCN2/CTGF is known to be involved in tooth germ development and periodontal tissue remodeling, as well as in mesenchymal tissue development and regeneration. In this present study, we investigated the roles of CCN2/CTGF in the proliferation and differentiation of periodontal ligament cells (murine periodontal ligament-derived cell line: MPL) in vitro. Results: In cell cultures of MPL, the mRNA expression of the CCN2/CTGF gene was stronger in sparse cultures than in confluent ones and was significantly enhanced by TGF-β. The addition of recombinant CCN2/CTGF (rCCN2) to MPL cultures stimulated DNA synthesis and cell growth in a dose-dependent manner. Moreover, rCCN2 addition also enhanced the mRNA expression of alkaline phosphatase (ALPase), type I collagen, and periostin, the latter of which is considered to be a specific marker of the periosteum and periodontium; whereas it showed little effect on the mRNA expression of typical osteoblastic markers, e.g., osteopontin and osteocalcin. Finally, rCCN2/CTGF also stimulated ALPase activity and collagen synthesis. Conclusion: These results taken together suggest important roles of CCN2/ CTGF in the development and regeneration of periodontal tissue including the periodontal ligament. © 2005 Asano et al; licensee BioMed Central Ltd.

引用

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