Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium: Prevention of cell death by AG126 and 15-deoxy-delta 12, 14-PGJ2

被引:75
作者
Tarun K Garg
Jason Y Chang
机构
[1] Department of Anatomy, Univ. of Arkansas for Med. Sciences, Little Rock
[2] Department of Ophthalmology, Univ. of Arkansas for Med. Sciences, Little Rock
关键词
Retinal Pigment Epithelium; Retinal Pigment Epithelium Cell; H2O2 Treatment; Photoreceptor Outer Segment; Human Retinal Pigment Epithelium Cell;
D O I
10.1186/1471-2415-3-5
中图分类号
学科分类号
摘要
Background: The retina, which is exposed to both sunlight and very high levels of oxygen, is exceptionally rich in polyunsaturated fatty acids, which makes it a favorable environment for the generation of reactive oxygen species. The cytotoxic effects of hydrogen peroxide (H2O2) induced)oxidative stress on retinal pigment epithelium were characterized in this study. Methods: The MTT cell viability assay, Texas-Red phalloidin staining, immunohistochemistry and Western blot analysis were used to assess the effects of oxidative stress on primary human retinal pigment epithelial cell cultures and the ARPE-19 cell line. Results: The treatment of retinal pigment epithelial cells with (H2O2 caused a dose-dependent decrease of cellular viability, which was preceded by a significant cytoskeletal rearrangement, activation of the Extracellular signal-Regulated Kinase, lipid peroxidation and nuclear condensation. This cell death was prevented partially by the prostaglandin derivative, 15d-PGJ 2 and by the protein kinase inhibitor, AG126. Conclusion: 15d-PGJ2 and AG126 may be useful pharmacological tools in the future capable of preventing oxidative stress induced RPE cell death in human ocular diseases.
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页码:1 / 15
页数:14
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