Escherichia coli isolated from seafood: Toxicity and plasmid profiles

被引：37

作者：

Teophilo G.N.D. ^{[2
]}

Vieira R.H.S.D.F. ^{[2
]}

Rodrigues D.D.P. ^{[1
]}

Menezes F.G.R. ^{[2
]}

机构：

[1] Fundação Oswaldo Cruz, Rio de Janeiro

[2] Instituto de Ciências do Mar - Labomar, Universidade Federal do Ceará, Meireles, Fortaleza 60165-081

来源：

International Microbiology | 2002年 / 5卷 / 1期

关键词：

Antimicrobial resistance; Enterotoxigenicity; Escherichia coli; Red snapper fish (Lutjanus purpureus); Seabob shrimp (Xiphopenaeus kroyeri);

D O I：

10.1007/s10123-002-0052-5

中图分类号：

学科分类号：

摘要：

Thirty-two Escherichia coli strains were isolated from red snapper (Lutjamus purpureus) and from seabob shrimp (Xiphopenaeus kroyeri). The strains were numbered S1-S16, and F1-F16, which corresponds to the isolation origin from shrimp (S) and fish (F). The isolates were biologically and antigenically characterized by agglutination tests with enteropathogenic E. coli (EPEC)-, enteroinvasive E. coli (EIEC)- and enterohemorrhagic E. coli (EHEC) O157-specific antisera. The ETEC enterotoxins were characterized by GMI-ELISA for enterotoxin LT-1 (thermolabile) and by inoculation of supernatants prepared from newly born mice for enterotoxin Sta. A total of 14 strains produced exotoxins, of which seven were thermolabile (LT) and seven were thermostable (ST). Antimicrobial susceptibility profiles were determined by disc diffusion in agar using ampicillin, cephalothin, cefoxitin, ceftriaxone, imipenem, nalidixic acid, ciprofloxacin, chloramphenicol, gentamicin, nitrofurantoin, sulfamethoxazole-trimethoprim, and tetracycline. Four isolates showed lower susceptibility to some antibiotics, two strains were resistant to ampicillin, tetracycline, and sulfamethoxazole-trimethoprim, and two were resistant to tetracycline and nitrofurantoin. Plasmids were extracted in the four resistant isolates; two of them contained plasmids whose molecular weight varied from low to high. The characterization of LT- and ST-toxin-producing E. coli strains displaying multiresistance and containing plasmids suggests the need for tightening current control measures for the use of antimicrobials. © Springer-Verlag and SEM 2002.

引用

页码：11 / 14

页数：3

共 22 条

[1]

Binsztein N., Rivas M., Moral L.L., Viboud G., Iriarte C., Szefner M., Svennerholm A.M., Relationship between enterotoxigenic Escherichia coli and diarrhea among children in Buenos Aires, Medicina (Buenos Aires), 52, pp. 103-108, (1992)

[2]

Birnboim H.C., Doly J., A rapid alkaline extration procedure for screening recombinant plasmid DNA, Nucleic Acid Res, 7, pp. 1513-1523, (1979)

[3]

Black R.E., Pathogens that cause traveler's diarrhea in Latin America and Africa, Rev Infect Dis, 8, pp. 131-135, (1986)

[4]

Brown D.J., Threffall E.J., Rowe B., Instability of multiple drug resistance plasmid, Epidemiol Infect, 106, pp. 247-257, (1991)

[5]

Castell C.H., Smith B., Dier W.J., Simultaneous measurements of trimethylamine and dimethylamine in fish, and their use for estimating quality of frozen-stored Gadoid fillets, J Fish Res Board Canada, 31, pp. 383-389, (1974)

[6]

Dean A.G., Ching Yi.-C., Williams R.G., Harden L.R., Test for Escherichia coli enterotoxin using infant mice: Application in a study of diarrhea in children in Honolulu, J Infect Dis, 125, pp. 407-411, (1972)

[7]

Ewing W.H., Differentiation of Enterobacteriaceae by biochemical reactions, Edward and Ewing's Identification of Enterobacteriaceae, 4th Edn., (1986)

[8]

Bacteriological Analytical Manual, 7th Edn., (1992)

[9]

Frischer M.E., Stewart G.J., Paul J.H., Plasmid transfer to indigenous marine bacterial populations by natural transformation, FEMS Microbiol Ecol, 15, pp. 127-136, (1994)

[10]

Lebaron Ph., Batailler N., Baleux B., Mobilization of a recombinant nonconjugative plasmid at the interface between wastewater and the marine coastal environment, FEMS Microbiol Ecol, 15, pp. 61-70, (1994)

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