Effects of 17β-estradiol on the expression of IL-6, IL-11 and NF-κB in human MG-63 osteoblast-like cell line

被引：2

作者：

Wang Yunlin

Liao Eyuan

Xiang Guangda

Dai Ruchun

Xiao Xinhua

Luo Xianghang

机构：

[1] Huazhong university of Science and Technology,Department of Gerontism, Tongji Hospital, Tongji Medical College

[2] Central South University,Institute of Endocrinology and Metabolism, Second Xiangya Hospital

[3] Wuhan General Hospital of PLA,Department of Endocrinology

来源：

Journal of Huazhong University of Science and Technology [Medical Sciences] | 2006年 / 26卷 / 1期

关键词：

IL-6; IL-11; NF κB; MG-63;

D O I：

10.1007/BF02828038

中图分类号：

学科分类号：

摘要：

In order to characterize the effects of 17β estradiol (17β-E2) on the expression of IL-6, IL 11 and NF-κB in the human MG 63 osteoblast like cell line, the expression of IL-6 was detected by RT-PCR. Northern blot and Western blot. The expression of IL-11 was determined by RT-PCR, and NF-κB by Western blot. The results showed that 17β-E2 down-regulated the expression of IL 6 mRNA and protein. IL 11 mRNA and NF κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG 63 cells could be suppressed by 17β-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.

引用

页码：53 / 58

页数：5

共 54 条

[1]

Lindsay R(1976)Longterm prevention of postmenopausal osteoporosis by estrogen: evidence for an increased bone mass after delayed onset of estrogen treatment Lancet 1 1038-1041

[2]

Hart D M(1988)Estrogens in the prevention and treatment of postmenopausal osteoporosis: a review Am J Med 85 847-850

[3]

Aitken J M(1988)Evidence of estrogen receptors in normal human osteoblast-like cells Science (Wash. DC) 241 84-86

[4]

Barzel U S(1991)Chinese hamster ovarian cells transfected with the murine interleukin-6 gene cause hypercalcemia as well as cachexia, leukocytosis and thrombocytosis in tumor-bearing nude mice Endocrinology 128 2657-2659

[5]

Eriksen E F(1995)Increased cytokine secretion by human bone marrow cells, after menopause or discontinuation of estrogen replacement J Clin Endocrinol Metab 80 3351-3355

[6]

Colvard D S(1997)Regulation of the gp80 and gp130 subunits of the IL-6 receptor by sex steroids in the murine bone marrow J Clin Invest 100 1980-1990

[7]

Berg N J(1998)Estrogen inhibits interleukin-1beta-induced interleukin-6 production by human osteoblast-like cells J Interferon Cytokine Res 18 479-483

[8]

Black K(2000)Estrogen modulates parathyroid hormone-induced interleukin-6 production Endocrinology 141 2526-2531

[9]

Garrett I R(1999) and J Bone Mineral Res 14 1330-1337

[10]

Mundy G R(1999)The anti-androgen hydroxyflutamide and androgens inhibit interleukin-6 production by an androgen-responsive human osteoblastic cell line Bone 25 211-215

← 1 2 3 4 5 6 →