Cell orientation induced by extracellular signals

被引:34
作者
Ralf Kemkemer
Cornelia Neidlinger-Wilke
Lutz Claes
Hans Gruler
机构
[1] Universität Ulm,BioPhysics Department, Complex Fluids Group
[2] Universität Ulm,Abteilung Unfallchirugische Forschung und Biomechanik
关键词
Fibroblast; Osteoblast; Guidance;
D O I
10.1007/BF02738066
中图分类号
学科分类号
摘要
Cells like fibroblasts and osteoblasts are oriented by different extracellular guiding signals like an electric field, a bent surface, and a periodically stretched surface. An automatic controller is responsible for the cell alignment. The controller contains both a deterministic and a stochastic signal. The following machine properties were determined:The angle dependence of the cellular signal transformer is cos {ie167-1}.The set point of the automatic controller is {ie167-2}. The cells like to orient their long axis perpendicular to the direction of the applied guiding signal.The signal transformer measures the extracellular signal in a quadratic fashion. The cells cannot register the sign of the guiding field.The stochastic signal in the automatic controller can be quantified by a characteristic time (≈ 130 min for fibroblasts).The extracellular signal is registered in cell-made standards (ratio of the deterministic and stochastic signal equals one): 0.3±0.05 V/mm for human fibroblasts (electric field) and 85±3 μm for human fibroblasts and osteoblasts (cylindrically bent surface).The lag-time in the signal transduction system of fibroblasts is ≈ 4 min.
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页码:167 / 192
页数:25
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共 43 条
  • [1] Schienbein M.(1994)Random walk and directed movement: comparison between inert particles and self-organized molecular machines Phys. Rev. E. 49 5492-5471
  • [2] Franke K.(1986)Applied electrical fields polarize the growth of mycelial fungi J. Gen. Microbiol. 132 2515-2525
  • [3] Gruler H.(1990)Directed growth of fungal hyphae in an electric field Z. Naturforsch. 45c 306-313
  • [4] McGillivray A. M.(1984)Embryonic fibroblast motility and orientation can be influenced by physiological fields J. Cell Biol. 98 296-307
  • [5] Gow N.A.R.(1989)Studies on the alignment of fibroblasts in uniform applied electric fields Bielectom. 10 371-384
  • [6] Gruler H.(1984)Perpendicular orientation and directional migration of amphibian neural crest cells in de electrical fields Proc. Natl. Acad. Sci. USA 81 160-164
  • [7] Gow N. A.(1986)Osteoclasts and Osteoblasts Migrate in Opposite Directions in Response to a Constant Electric Field J. Cell Physiol. 129 283-288
  • [8] Erickson C.A.(1994)Cyclic stretching of human osteoblasts affects proliferation and metabolism: A new experimental method and its application J. Orthop. Res. 12 70-78
  • [9] Nuccitelli R.(1988)Physiological electric fields can influence cell motility, growth, and polarity Adv. Cell Biol. 2 213-233
  • [10] Ross S.M.(1995)Chemical amplifier, self-ignition mechanism and amoeboid cell migration Phys. Rev. E. 52 4183-4197