Gene expression profiling of potato responses to cold, heat, and salt stress

被引：104

作者：

Rensink W.A. ^{[1
]}

Iobst S. ^{[1
]}

Hart A. ^{[1
]}

Stegalkina S. ^{[1
]}

Liu J. ^{[1
]}

Buell C.R. ^{[1
]}

机构：

[1] The Institute for Genomic Research, Rockville, MD 20850

来源：

Functional & Integrative Genomics | 2005年 / 5卷 / 4期

基金：

美国国家科学基金会;

关键词：

Abiotic stress; Gene expression profiling; Potato;

D O I：

10.1007/s10142-005-0141-6

中图分类号：

学科分类号：

摘要：

In order to identify genes involved in abiotic stress responses in potato, seedlings were grown under controlled conditions and subjected to cold (4°C), heat (35°C), or salt (100 mM NaCl) stress for up to 27 h. Using an ∼12,000 clone potato cDNA microarray, expression profiles were captured at three time points following initiation of the stress (3, 9, and 27 h) from two different tissues, roots and leaves. A total of 3,314 clones could be identified as significantly up- or down-regulated in response to at least one stress condition. The genes represented by these clones encode transcription factors, signal transduction factors, and heat-shock proteins which have been associated with abiotic stress responses in Arabidopsis and rice, suggesting similar response pathways function in potato. These stress-regulated clones could be separated into either stress-specific or shared-response clones, suggesting the existence of general response pathways as well as more stress-specific pathways. In addition, we identified expression profiles which are indicative for the type of stress applied to the plants. © Springer-Verlag 2005.

引用

页码：201 / 207

页数：6

共 27 条

[1]

Cui X., Churchill G.A., Statistical tests for differential expression in cDNA microarray experiments, Genome Biol., 4, (2003)

[2]

Dudoit S., Gentleman R.C., Quackenbush J., Open source software for the analysis of microarray data, BioTechniques, SUPPL., pp. 45-51, (2003)

[3]

Fowler S., Thomashow M.F., Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway, Plant Cell, 14, pp. 1675-1690, (2002)

[4]

Hazen S.P., Wu Y., Kreps J.A., Gene expression profiling of plant responses to abiotic stress, Funct. Integr. Genomics, 3, pp. 105-111, (2003)

[5]

Hegde P., Qi R., Abernathy K., Gay C., Dharap S., Gaspard R., Hughes J.E., Snesrud E., Lee N., Quackenbush J., A concise guide to cDNA microarray analysis, BioTechniques, 29, pp. 548-554, (2000)

[6]

Iba K., Acclimative response to temperature stress in higher plants: Approaches of gene engineering for temperature tolerance, Annu. Rev. Plant Biol., 53, pp. 225-245, (2002)

[7]

Ihaka R., Gentleman R., R: A language for data analysis and graphics, J. Comput. Graph. Stat., 5, pp. 299-314, (1996)

[8]

Kawasaki S., Borchert C., Deyholos M., Wang H., Brazille S., Kawai K., Galbraith D., Bohnert H.J., Gene expression profiles during the initial phase of salt stress in rice, Plant Cell, 13, pp. 889-905, (2001)

[9]

Kerr M.K., Churchill G.A., Bootstrapping cluster analysis: Assessing the reliability of conclusions from microarray experiments, Proc. Natl. Acad. Sci. U. S. A., 98, pp. 8961-8965, (2001)

[10]

Kreps J.A., Wu Y., Chang H.S., Zhu T., Wang X., Harper J.F., Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress, Plant Physiol., 130, pp. 2129-2141, (2002)

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