Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon

被引：11

作者：

Dantec L.L. ^{[1
]}

Bové J.M. ^{[1
]}

Saillard C. ^{[1
]}

机构：

[1] Lab. de Biol. Cell. et Molec., Inst. Natl. de la Rech. Agronomique, Univ. Victor Segalen Bordeaux 2, BP 81, 33883 Villenave d'Ornon Cedex, Domaine de la Grande Ferrade

来源：

Current Microbiology | 1998年 / 37卷 / 4期

关键词：

Nucleotide; Nucleotide Sequence; Pyruvate; Northern Blot; Ribosomal Protein;

D O I：

10.1007/s002849900377

中图分类号：

学科分类号：

摘要：

The nucleotide sequence of a 6863-bp Spiroplasma citri DNA fragment comprising the spiralin gene was determined. Sequence analysis revealed eight putative ORFs that encode ribosomal protein S2, elongation factor Ts, spiralin, 6-phosphofructokinase, pyruvate kinase, and three unidentified proteins (A, B, and X). The gene organization reported here is different from that previously published. Northern blot analysis of rpsB, tsf, and x transcripts indicates that these genes are organized into a single transcriptional unit (operon). However, the detection of an additional transcript corresponding to the rpsB gene alone suggests that a transcriptional mechanism should occur in the 3' region of the rpsB gene, allowing a conditional transcription termination.

引用

页码：269 / 273

页数：4

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