Partial outlet obstruction of the rat bladder induces a stimulatory response on proliferation of the bladder smooth muscle cells

被引：19

作者：

Hanai T. ^{[1
]}

Ma F.-H. ^{[2
]}

Matsumoto S. ^{[1
]}

Park Y.C. ^{[1
]}

Kurita T. ^{[1
]}

机构：

[1] Department of Urology, Kinki University, School of Medicine, Osaka-Sayama, Osaka 589-8511

[2] Research Laboratories, Nippon Shinyaku Co. Ltd., Kyoto

来源：

International Urology and Nephrology | 2002年 / 34卷 / 1期

关键词：

Bladder outlet obstruction; Cultured smooth muscle cells; Growth factors; Rat;

D O I：

10.1023/A:1021398626846

中图分类号：

学科分类号：

摘要：

Outlet obstruction of the rat bladder induces hypertrophy/hyperplasia characterized by increases in bladder mass, smooth muscle content, and collagen deposition. In order to understand the mechanism of the outlet obstruction-induced hypertrophy and hyperplasia, we first determined the temporal pattern of changes in bladder mass after inducing the outlet obstruction. Histological analysis revealed that the smooth muscle cells with hypertrophy and hyperplasia, fibroblasts and connective tissue were increased in a time-dependent manner, corresponding to the temporal pattern observed in the changes in bladder mass, although the phase of changes in these tissue components was somewhat different. In order to further determine whether any proliferation-stimulatory factors were released from the bladder with obstruction in correspondence with increased bladder mass, soluble fractions were prepared from the bladders with outlet obstruction for 3-30 weeks, and their effects on proliferation of smooth muscle cells were examined. The soluble fractions prepared from the bladders at 3 to 14 weeks after obstruction slightly but significantly facilitated the proliferation of cultured smooth muscle cells, while the soluble fractions released after 20 weeks rather suppressed the proliferation. These results suggest that the initial increase in bladder mass might be in part due to the facilitated proliferation of smooth muscle cells of the bladder body induced by growth factors released into the soluble fractions, and that hypertrophy might then play a role in the increased bladder mass at later phases.

引用

页码：37 / 42

页数：5

共 14 条

[1]

Ahmed S.A., Global R.M. Jr., Walsh J.E., A new rapid and simple nonradioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to <sup>3</sup>H-thymidine incorporation assay, J Immun Method, 170, pp. 211-224, (1994)

[2]

Buttyan R., Jacob B.Z., Blavivas J.G., Levin R.M., Early molecular response to rabbit bladder obstruction, Neurol Urodyn, 11, pp. 225-238, (1992)

[3]

Buttyan R., Chen M.-W., Levin R.M., Animal models of bladder outlet obstruction and molecular insights into the basis for the development of bladder dysfunction, Eur Urol, 32, SUPPL. 4, pp. 32-39, (1997)

[4]

Gosling J.A., Gilpin S.A., Dixon J.S., Glipin C.J., Decrease in the autonomic innervation of human detrusor muscle in outflow obstruction, J Urol, 136, pp. 501-504, (1986)

[5]

Kato K., Monson F.C., Longhurst P.A., Wein A.J., Hauggard N., Levin R.M., The functional effects of long-term outlet obstruction on the rabbit urinary bladder, J Urol, 143, pp. 600-606, (1990)

[6]

Kim J.C., Yoon J.Y., Seo S.I., Hwang T.K., Park Y.H., Effects of partial bladder outlet obstruction and its relief on type I and III collagen and detrusor contractility in the rat, Neurol Urodyn, 19, pp. 29-42, (2000)

[7]

Kondo A., Susset J.A., Collagen content in detrusor muscle and ratio of bladder weight to body weight, Acta Urol Jap, 19, pp. 683-687, (1973)

[8]

Linder P., Mattiasson A., Persson L., Uvelius B., Reversibility of detrusor hypertrophy and hyperplasia after removal of infravesical outflow obstruction, J Urol, 140, pp. 642-646, (1988)

[9]

Ma F.-H., Higashira U., Ukai Y., Hanai T., Kiwamato H., Park Y.C., Et al., A new enzymic method for the isolation and culture of human bladder body smooth muscle cells, Neurol Urodyn

[10]

Ma F.-H., Higashira H., Ukai Y., Yoshikuni Y., Kimura K., Culture of rat bladder body smooth muscle cells by an enzymatic method and their binding properties for muscarinic and β-adrenergic receptor antagonist, Jpn J Pharmacol, 71, SUPPL. 1, (1996)

← 1 2 →