Hpa1 secretion via type III secretion system in Xanthomonas oryzae pv. oryzae

被引：33

作者：

Ayako Furutani

Seiji Tsuge

Takashi Oku

Kazunori Tsuno

Yasuhiro Inoue

Hirokazu Ochiai

Hisatoshi Kaku

Yasuyuki Kubo

机构：

[1] Kyoto Prefectural University,Laboratory of Plant Pathology, Graduate School of Agriculture

[2] Hiroshima Prefectural University,Laboratory of Molecular Plant Pathology, School of Bioresources

[3] Miyazaki University,Faculty of Agriculture

[4] National Institute of Agrobiological Sciences,undefined

来源：

Journal of General Plant Pathology | 2003年 / 69卷 / 4期

关键词：

pv. ; Type III secretion system; Hpa1;

D O I：

10.1007/s10327-003-0042-2

中图分类号：

学科分类号：

摘要：

In many Gram-negative plant pathogenic bacteria the type III secretion system (TTSS), encoded by hrp genes, is essential for pathogenicity in the host and induction of a hypersensitive reaction (HR) in nonhost plants. The expression of hrp genes has been suggested to be repressed in complex media, whereas it is induced in planta and under certain in vitro conditions. We recently reported that XOM2 medium allows efficient hrp expression by Xanthomonas oryzae pv. oryzae. In this study, we investigated hrp-dependent secretion of proteins by the bacteria in vitro. Using modified XOM2, in which bovine serum albumin was added and the pH was lowered to 6.0, we detected at least 10 secreted proteins and identified one as Hpa1. This is the first evidence of protein secretion via TTSS in X. oryzae pv. oryzae.

引用

页码：271 / 275

页数：4