HPLC determination of phytoplankton pigments using N,N-dimethylformamide

被引：100

作者：

Furuya K. ^{[1
]}

Hayashi M. ^{[1
]}

Yabushita Y. ^{[2
,3
]}

机构：

[1] Department of Aquatic Bioscience, Grad. Sch. of Agric. and Life Sci., University of Tokyo, Yayoi, Bunkyo

[2] Faculty of Bioresources, Mié University, Kamihama, Tsu

[3] Akagi Suisan Co., 3211, Handa, Shibukawa

来源：

Journal of Oceanography | 1998年 / 54卷 / 2期

关键词：

Chlorophyll a; HPLC; N; N-dimenthylformamide; Phytoplankton; Pigments;

D O I：

10.1007/BF02751695

中图分类号：

学科分类号：

摘要：

The suitability of N,N-dimethylformamide (DMF) as an extractant for the standard reverse-phase HPLC method was examined using algal cultures. Good pigment separations and recovery were achieved with 20% (volume %) addition of an ion-pairing solution in an injection. While slight amounts of degradation products of chlorophyll a, i.e., chlorophyllide a, allomeric and epimeric forms, were produced, adequate attention to filtration and extraction prevents the formation of degradation products, confining them to an acceptable level. Because of its strong extractability, which expedites the extraction process, DMF is an efficient solvent for HPLC analysis of phytoplankton pigments.

引用

页码：199 / 203

页数：4

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