Ultrastructural and cytochemical characterization of human cord blood cells

被引：14

作者：

Mikami T. ^{[1
]}

Eguchi M. ^{[1
]}

Kurosawa H. ^{[1
]}

Sato Y. ^{[1
]}

Sugita K. ^{[1
]}

Suzumura H. ^{[1
]}

Tadokoro N. ^{[2
]}

Watanabe H. ^{[2
]}

Inaba N. ^{[2
]}

机构：

[1] Department of Pediatrics, Dokkyo University School of Medicine, Tochigi 321-0293, 880 Kitakobayashi, Mibu-machi

[2] Department of Obstetrics and Gynecology, Dokkyo University School of Medicine, Tochigi, Mibu-machi

来源：

Medical Electron Microscopy | 2002年 / 35卷 / 2期

关键词：

Bone marrow; Cytochemistry; Electron microscopy; Human cord blood; Myeloperoxidase reaction; Stem cell;

D O I：

10.1007/s007950200012

中图分类号：

学科分类号：

摘要：

As part of a study to identify the characteristics of cord blood cells, we examined their morphological features by electron microscopy. Additionally, we cultured CD34-positive cells derived from cord blood and from bone marrow to perform morphological observations, as well as cytochemical examinations following the peroxidase reaction. Compared with normal peripheral blood cells, cord blood cells frequently showed immature morphology and a unique ultrastructure, such as nuclear pockets in neutrophils, several crystalloids in a single eosinophilic granule, and deformed nuclei in lymphoctytes. In contrast to bone marrow cells, cord blood cells yielded a large number of cells of immature myelo-monocytic lineages in cell culture, and demonstrated a weaker peroxidase reaction. We identified that cord blood cells were different from normal peripheral blood cells and bone marrow cells, confirming the functional differences that were previously assumed.

引用

页码：96 / 101

页数：5

共 18 条

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