A co-fermentation strategy to consume sugar mixtures effectively

被引：110

作者：

Eiteman M.A. ^{[1
]}

Lee S.A. ^{[1
]}

Altman E. ^{[1
]}

机构：

[1] Center for Molecular BioEngineering, Department of Biological and Agricultural Engineering, University of Georgia, Athens

来源：

Journal of Biological Engineering | / 2卷 / 1期

关键词：

Xylose; Lignocellulosic Hydrolysate; Xylose Isomerase; Xylose Consumption; Sugar Mixture;

D O I：

10.1186/1754-1611-2-3

中图分类号：

学科分类号：

摘要：

We report a new approach for the simultaneous conversion of xylose and glucose sugar mixtures into products by fermentation. The process simultaneously uses two substrate-selective strains of Escherichia coli, one which is unable to consume glucose and one which is unable to consume xylose. The xylose-selective (glucose deficient) strain E. coli ZSC113 has mutations in the glk, ptsG and manZ genes while the glucose-selective (xylose deficient) strain E. coli ALS1008 has a mutation in the xylA gene. By combining these two strains in a single process, xylose and glucose are consumed more quickly than by a single-organism approach. Moreover, we demonstrate that the process is able to adapt to changing concentrations of these two sugars, and therefore holds promise for the conversion of variable sugar feed streams, such as lignocellulosic hydrolysates. © 2008 Eiteman et al; licensee BioMed Central Ltd.

引用

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