Nuclear localization of SP, CGRP, and NK1R in a subpopulation of dorsal root ganglia subpopulation cells in rats

被引：16

作者：

Patrícia Aline Boer ^{[1
]}

José Antonio Rocha Gontijo ^{[1
]}

机构：

[1] Laboratório Balanço Hidro-Salino, Núcleo de Medicina e Cirurgia Experimental, Departamento de Clínica Médica, Universidade Estadual de Campinas, 13083-970, SP

来源：

Cellular and Molecular Neurobiology | 2006年 / 26卷 / 2期

基金：

巴西圣保罗研究基金会;

关键词：

CGRP; Dorsal root ganglia; Substance P; Tachykinin NK1 receptor;

D O I：

10.1007/s10571-006-9020-5

中图分类号：

学科分类号：

摘要：

Signals generated by renal pelvic afferent nerves in response to stimulation are transmitted from peripheral processes of dorsal root ganglia neurons to their central terminals in the dorsal horn of the spinal cord to cause the release of neuropeptides, including SP and CGRP. All of the cellular activities of SP are considered to be mediated through interaction with NK 1R located on the cell surface. We have investigated the colocalization and subcellular distribution of NK1R, SP, and CGRP in different subpopulations of neurons that innervate renal tissue. Our findings therefore provide the first evidence for the presence of NK1R, SP, and CGRP in the nuclei of DGR neural cells. The physiological significance of this localization remains unknown. One possibility is that pelvic sensory neurons may regulate their responses to different stimuli by modulating the ratio of CGRP and SP release and/or nuclear NK1R expression. © 2006 Springer Science+Business Media, Inc.

引用

页码：191 / 207

页数：16

共 63 条

[1]

Abrenica B., Gilchrist J.S., Nucleoplasmic Ca(2+)loading is regulated by mobilization of-perinuclear Ca(2+), Cell Calcium, 28, pp. 127-136, (2000)

[2]

Aline Boer P., Ueno M., Sant'ana J.S., Saad M.J., Gontijo J.A.R., Expression and localization of NK<sub>1</sub>R, substance P and CGRP are altered in dorsal root ganglia neurons of spontaneously hypertensive rats (SHR), Mol. Brain Res., 138, pp. 35-44, (2005)

[3]

Alvarez F.J., Morris H.R., Priestley J.V., Sub-populations of smaller diameter trigeminal primary afferent neurons defined by expression of calcitonin gene-related peptide and the cell surface oligosaccharide recognized by monoclonal antibody LA4, J. Neurocytol., 20, pp. 716-731, (1991)

[4]

Baldassare J.J., Jarpe M.B., Alferes L., Raben D.M., Nuclear translocation of RhoA mediates the mitogen-induced activation of phospholipase D involved in nuclear envelope signal transduction, J. Biol. Chem., 272, pp. 4911-4914, (1997)

[5]

Belcheva M., Barg J., Rowinski J., Clark W.G., Gloeckner C.A., Ho A., Et al., Novel opioid binding sites associated with nuclei of NG108-15 neurohybrid cells, J. Neurosci., 13, pp. 104-114, (1993)

[6]

Bhattacharya M., Peri K.G., Almazan G., Ribeiro-da-Silva A., Shichi H., Durocher Y., Et al., Nuclear localization of prostaglandin E2 receptors, Proc. Natl. Acad. Sci., 95, pp. 15792-15797, (1998)

[7]

Bhattacharya M., Peri K., Ribeiro-da-Silva A., Almazan G., Shichi H., Hou X., Et al., Localization of functional prostaglandin E2 receptors EP3 and EP4 in the nuclear envelope, J. Biol. Chem., 274, pp. 15719-15724, (1999)

[8]

Boivin B., Chevalier D., Villeneuve L.R., Rousseau E., Allen B.G., Functional endothelin receptors are present on nuclei in cardiac ventricular myocytes, J. Biol. Chem., 278, pp. 29153-29163, (2003)

[9]

Booz G.W., Conrad K.M., Hess A.L., Singer H.A., Baker K.M., Angiotensin-II-binding sites on hepatocyte nuclei, Endocrinology, 130, pp. 3641-3649, (1992)

[10]

Bouche G., Gas N., Prats H., Baldin V., Tauber J.P., Teissie J., Et al., Basic fibroblast growth factor enters the nucleolus and stimulates the transcription of ribosomal genes in ABAE cells undergoing G0-G1 transition, Proc. Natl. Acad. Sci. USA, 84, pp. 6770-6774, (1987)

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