Rapid extraction of genomic DNA from leaves and bracts of dove tree (Davidia involucrata)

被引：6

作者：

Li Y.X. ^{[1
,2
]}

Su Z.X. ^{[2
]}

Chen F. ^{[1
]}

机构：

[1] College of Life Science, Sichuan University, Chengdu, Sichuan

[2] Department of Biology, Sichuan Teachers College, Nanchong, Sichuan

来源：

Plant Molecular Biology Reporter | 2002年 / 20卷 / 2期

关键词：

Davidia; DNA extraction; Matured tissues; Polyphenols;

D O I：

10.1007/BF02799433

中图分类号：

学科分类号：

摘要：

Large amounts of polyphenolics in dove tree leaves make it difficult to obtain high-quality genomic DNA during extraction. A rapid DNA minipreparation method was developed for dove tree (Davidia involucrata) and yields 40-50 μg genomic DNA from 0.1 g fresh matured and young leaves and bracts. The yield and quality of the resulting DNA is satisfactory, and the protocol can be scaled up according to sample size. The obtained DNA is suitable for PCR and the restriction enzyme digestion needed for Southern blotting.

引用

页码：185a / 185e

共 5 条

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[5]

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