Plant DNA extraction using silica

被引：15

作者：

Steven H. Rogstad

机构：

[1] Dept. of Biological Sciences ML6, University of Cincinnati, Cincinnati

来源：

Plant Molecular Biology Reporter | 2003年 / 21卷 / 4期

关键词：

DNA extraction; DNA purification; DNA sequencing; PCR; Restriction enzyme; Silicon dioxide;

D O I：

10.1007/BF02772598

中图分类号：

学科分类号：

摘要：

Described here is a method that uses silicon dioxide (silica) to extract whole genomic plant DNA of high molecular weight. The protocol is presented in a microcentrifuge format, and yields were approximately 2-4 μg per 200 mg of plant leaf tissue. The method involves fewer steps than many previous extraction protocols and, as shown here for 4 taxonomically distant angiosperms, produces DNA suitable for digestion with restriction endonucleases. The use of commercial kits is not required; the silica costs are comparatively inexpensive (>$0.03 per tube); and CTAB, rather than the more expensive guanidine thiocyanate salt, is used.

引用

页码：463a / 463g

共 28 条

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