Improved Technique for Isolating RNA from Tobacco Tissues

被引：12

作者：

Wang M.-H. ^{[1
]}

Rhee H.-I. ^{[1
]}

机构：

[1] Division of Food and Biotechnology, Kangwon National University, Chuncheon, Kangwon

来源：

Plant Molecular Biology Reporter | 2001年 / 19卷 / 2期

关键词：

Lithium chloride; RNA; Tobacco tissue;

D O I：

10.1007/BF02772164

中图分类号：

学科分类号：

摘要：

We have developed a much-improved method for isolating RNA from tobacco tissue. The novel component of the described RNA isolation method is the addition of lithium chloride to the extraction buffer. Following that, the RNA was homogenized with phenol/chloroform and precipitated in ethanol. This isolation technique provided highly reproducible and good quality RNA within 2 h.

引用

页码：187a / 187f

共 7 条

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