The toxicity of antibiotics and herbicides on in vitro adventitious shoot formation on Pinus pinea L. cotyledons

被引：21

作者：

Humara J.M. ^{[1
,2
]}

Ordás R.J. ^{[1
,2
]}

机构：

[1] Departamento de Biología de Organismos y Sistemas, Unidad de Fisiología Vegetal, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n, Oviedo, Asturias

来源：

In Vitro Cellular & Developmental Biology - Plant | 1999年 / 35卷 / 4期

关键词：

Glufosinate; Phosphinothricin; Ticarcillin; Tissue culture; Transformation;

D O I：

10.1007/s11627-999-0045-6

中图分类号：

学科分类号：

摘要：

The toxicity of three antibiotics commonly used to eliminate Agrobacterium tumefaciens from plant tissue during transformation were tested to determine their effect on Pinus pinea L. morphogenesis. Cefotaxime and vancomycin at a dose of 250 μg ml-1, as well as ticarcillin at 300 μg ml-1, were essentially nontoxic to the culture and significantly enhanced regeneration and shoot development. Kanamycin, a widely used selection agent for plant transformation, strongly inhibited regeneration even at very low doses. On the contrary, phosphinothricin and its ammonium salt glufosinate in the commercial formulation Finale® proved to be the best selection agents because they were less toxic to stone pine cotyledons. Thus, they could be applied immediately after transformation to avoid escapes. Other schemes for selection and recovery of transgenic stone pine trees are discussed.

引用

页码：339 / 343

页数：4

共 42 条

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