Feasibility of colloidal silver SERS for rapid bacterial screening

被引：12

作者：

Liu Y. ^{[1
]}

Chao K. ^{[1
]}

Nou X. ^{[1
]}

Chen Y.-R. ^{[1
]}

机构：

[1] Food Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, USDA, Beltsville, MD 20705

来源：

Sensing and Instrumentation for Food Quality and Safety | 2009年 / 3卷 / 02期

关键词：

E; coli; Food safety; Listeria; Salmonella; SERS; Silver colloid;

D O I：

10.1007/s11694-008-9064-y

中图分类号：

学科分类号：

摘要：

This study reports the feasibility of citrate-reduced colloidal silver surface-enhanced Raman scattering (SERS) for differentiating three important food borne pathogens, E. coli, Listeria, and Salmonella. FT-Raman and SERS spectra of both silver colloids and colloid-K 3PO 4 mixtures were collected and analyzed to evaluate the reproducibility and stability of silver colloids fabricated in a batch-production process. The results suggest that the reproducibility of the colloids over the batch process is high and that their binding effectiveness remains consistent over a 60-day storage period. Two specific SERS bands at 712 and 390 cm -1 were identified and used to develop simple 2-band ratios for differentiating E. coli-, Listeria-, and Salmonella-colloid mixtures with a 100% success. These results indicate that colloidal silver SERS technique may be a practical alternative method suitable for routine and rapid screening of E. coli, Listeria, and Salmonella bacteria. © Springer Science+Business Media, LLC 2008.

引用

页码：100 / 107

页数：7

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