Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

被引：499

作者：

Vences M. ^{[1
]}

Thomas M. ^{[2
]}

Van Der Meijden A. ^{[3
]}

Chiari Y. ^{[3
]}

Vieites D.R. ^{[4
]}

机构：

[1] Inst. Biodiv. and Ecosystem Dynam., Zoological Museum, University of Amsterdam, 1092 AD Amsterdam

[2] Institute for Genetics, Evolutionary Genetics, University of Cologne, 50931 Köln

[3] Department of Biology, University of Konstanz

[4] Department of Integrative Biology, Museum of Vertebrate Zoology, University of California, Berkeley

来源：

Frontiers in Zoology | / 2卷 / 1期

关键词：

Amphibian Species; Haplotype Sharing; Advertisement Call; Universal Applicability; Local Blast Search;

D O I：

10.1186/1742-9994-2-5

中图分类号：

学科分类号：

摘要：

Background: Identifying species of organisms by short sequences of DNA has been in the center of ongoing discussions under the terms DNA barcoding or DNA taxonomy. A C-terminal fragment of the mitochondrial gene for cytochrome oxidase subunit I (COI) has been proposed as universal marker for this purpose among animals. Results: Herein we present experimental evidence that the mitochondrial 16S rRNA gene fulfills the requirements for a universal DNA barcoding marker in amphibians. In terms of universality of priming sites and identification of major vertebrate clades the studied 16S fragment is superior to COI. Amplification success was 100% for 16S in a subset of fresh and well-preserved samples of Madagascan frogs, while various combination of COI primers had lower success rates. COI priming sites showed high variability among amphibians both at the level of groups and closely related species, whereas 16S priming sites were highly conserved among vertebrates. Interspecific pairwise 16S divergences in a test group of Madagascan frogs were at a level suitable for assignment of larval stages to species (1-17%), with low degrees of pairwise haplotype divergence within populations (0-1%). Conclusion: We strongly advocate the use of 16S rRNA as standard DNA barcoding marker for vertebrates to complement COI, especially if samples a priori could belong to various phylogenetically distant taxa and false negatives would constitute a major problem. © 2005 Vences et al; licensee BioMed Central Ltd.

引用

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