Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 · 7H2O aqueous two-phase system to produce nisin

被引：6

作者：

Chan Li

Fan Ouyang

Jinghua Bai

机构：

[1] Chinese Academy of Sciences,State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy

[2] Hubei University,School of Life Science

来源：

Biotechnology Letters | 2000年 / 22卷

关键词：

aqueous two-phase systems; extractive cultivation; lactic acid; nisin;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

To relieve lactic acid inhibition, an aqueous two-phase system (ATPS) was used to grow Lactococcus lactis. Its composition was 11% (w/v) PEG 20000/3.5% (w/v) MgSO4 ⋅ 7H2O. In this ATPS medium, the cells were completely partitioned in the bottom phase, and lactic acid had the biggest partition coefficient of the eight ATPS media tested. The cell biomass in this medium was 0.64 mg ml−1, only 60% of that of the control medium, but nisin production (803 IU ml−1) was enhanced by 33%. The increase in nisin was explained as a result of extraction of lactic acid from the bottom phase to the top one. The changes of tie-line length and phase volume ratio for the identical tie line could affect cell growth and nisin accumulation.

引用

页码：843 / 847

页数：4

共 19 条

[1]

Dissing U(1994)Cultivation of Biotechnol. Lett. 16 333-338

[2]

Mattiasson B(1997) in a polyelectrolyte neutral polymer aqueous two-phase system J. Chem. Tech. Biotechnol. 69 5-10

[3]

Hotha S(1995)Production of alkaline protease by Biotechnol. Prog. 11 202-207

[4]

Banik RM(1980) H14 in aqueous two-phase systems Biotechnol. Bioeng. 22 2393-2398

[5]

Kuboi R(1990)Extractive cultivation of J. Ferment. Bioeng. 69 82-89

[6]

Umakoshi H(1960) using poly(ethylene glycol)/phosphate aqueous two-phase systems to produce intracellular Milchwissenschaft 15 7-11

[7]

Komasawa I(1997)-galactosidase Int. J. Food Microbiol. 36 1-29

[8]

Kuhn I(1964)Alcohol fermentation in an aqueous two-phase system J. Sci. Food Agric. 15 522-528

[9]

Lee YH(1992)Production of alkaline protease by Appl. Environ. Microbiol. 58 3355-3359

[10]

Chang HN(undefined) in an aqueous two-phase system undefined undefined undefined-undefined

← 1 2 →