Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in Phomopsis Isolates from Fruit Trees

被引:24
作者
Satoko KANEMATSU
Nobuhiro MINAKA
Takao KOBAYASHI
Akira KUDO
Yoshihiro OHTSU
机构
[1] National Institute of Fruit Tree Science,
[2] Tsukuba 305–8605,undefined
[3] Japan,undefined
[4] Apple Research Center,undefined
[5] National Institute of Fruit Tree Science,undefined
[6] Morioka 020–0123,undefined
[7] Japan,undefined
[8] National Institute of Agro-Environmental Sciences,undefined
[9] Tsukuba 305–8604,undefined
[10] Japan,undefined
[11] Department of International Agricultural Development,undefined
[12] Tokyo University of Agriculture,undefined
[13] Setagaya-ku,undefined
[14] Tokyo 156–8502,undefined
[15] Japan,undefined
关键词
Key words : Diaporthe eres, Diaporthe medusaea, Phomopsis amygdali, rDNA ITS regions, mating, taxonomy.;
D O I
10.1007/PL00012944
中图分类号
学科分类号
摘要
Sequences of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) regions were examined to infer a molecular phylogeny of small-spored Phomopsis isolates, designated W-type (mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25°C on PDA) and G-type (mainly gray colony, highly virulent, bearing only alpha conidia at 25°C on PDA), and P. amygdali from fruit trees. Phomopsis G-type and P. amygdali were a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups. Diaporthe citri, D. tanakae, P. asparagi, P. viticola, P. vitimegaspora and D. nomurai, which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of the Phomopsis species and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type and P. amygdali collected in Japan were cross-fertile. Some isolates from Lunaria annua, Ulmus glabra and Juglans regia belonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit trees.
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页码:191 / 201
页数:10
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