Role of insulin-like growth factor binding protein-4 in prevention of colon cancer

被引：16

作者：

Durai R. ^{[1
,2
]}

Yang S.Y. ^{[1
,2
]}

Seifalian A.M. ^{[1
,2
]}

Goldspink G. ^{[1
]}

Winslet M.C. ^{[1
,2
]}

机构：

[1] Academic Division of Surgical and Interventional Sciences, University College London, London

[2] Royal Free Hampstead NHS Trust Hospital, London

来源：

World Journal of Surgical Oncology | / 5卷 / 1期

关键词：

Colon Cancer; Familial Adenomatous Polyposis; TUNEL Assay; Apoptotic Index; Secondary Antibody Conjugate;

D O I：

10.1186/1477-7819-5-128

中图分类号：

学科分类号：

摘要：

Background: Insulin-like growth factors (IGFs) are important for the proliferation of cancer cells. One of their binding proteins, known as insulin-like growth factor binding protein -4 (IGFBP-4) is well known for its inhibitory action on IGFs in vitro. We assessed the effect of IGFBP-4 in prevention of development of colon cancer in vivo. Methods: Nude mice were subcutaneously inoculated with HT-29 colon cancer cells and they were also simultaneously injected either gene construct containing mammalian expression vector pcDNA3 with or without IGFBP-4 gene or phosphate buffered saline. The effect was assessed 4 weeks later by evaluating the tumours for mitosis, necrosis, apoptosis, and expressions of IGFBP-4, Bcl-2 and Bax proteins. Results: The results showed that the IGFBP-4 gene therapy did not prevent the tumour establishment but it increased the tumour apoptosis which was associated with an increase in Bcl-2 and Bax expressions. The IGFBP-4 protein was low in tumours which received IGFBP-4 gene construct which may be due to a feed back mechanism of IGFBP-4 upon its own cells. Conclusion: IGFBP-4 gene therapy in the form localised gene transfer did not prevent colon cancer initiation and establishment but it resulted in increased apoptosis and Bax protein expression and a decrease in tumour cellular mitosis. © 2007 Durai et al; licensee BioMed Central Ltd.

引用

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