Myocardial oxidative stress and antioxidants in hypertension as a result of nitric oxide synthase inhibition

被引：10

作者：

Belló-Klein A. ^{[1
,2
,4
]}

Bock P.M. ^{[1
]}

Travacio M. ^{[3
]}

Senna S.M. ^{[1
]}

Llesuy S. ^{[3
]}

de Bittencourt P.I.H. ^{[1
]}

Jr. ^{[1
]}

Irigoyen M.C. ^{[1
]}

Bello A.A. ^{[1
]}

Kumar D. ^{[2
,4
]}

Singal P.K. ^{[2
,4
]}

机构：

[1] Laboratório de Fisiologia Cardiovascular, Departmento de Fisiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS

[2] Department of Physiology, Faculty of Medicine, The University of Manitoba, Winnipeg

[3] Catedra de Fisicoquimica, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires

[4] Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, MB, R2H 2A6, 351 Tache Ave.

来源：

Cardiovascular Toxicology | 2001年 / 1卷 / 1期

关键词：

Free radicals; hypertension; nitro-; -arginine; antioxidant enzymes;

D O I：

10.1385/CT:1:1:43

中图分类号：

学科分类号：

摘要：

Rats were made hypertensive by the administration of the nitric oxide synthase inhibitor nitro-L-arginine (LNA, 2.74 mmol/L) in drinking water for 7 d. Hearts from hemodynamically assessed animals were analyzed for lipid peroxidation (LPO), gamma-glutamylcysteine-synthetase (gamma-GCS), glutathione disulfide reductase (GR), glutathione peroxidase (GSHPx), catalase (CAT), superoxide dismutase (SOD), and total radical trapping potential (TRAP) activities. LNA treatment increased the mean arterial blood pressure by 46% and the heart rate by 22% without changing plasma renin activity. LNA treatment resulted in a 30% increase in LPO. gamma-GCS was reduced by 48% and GR by 36% in the cardiac tissue of hypertensive rats as compared to controls. The activity of nonselenium GSHPx was reduced by 27%, and selenium-dependent GSHPx activity in the heart was not affected by LNA treatment. In hypertensive rats, SOD activity was increased by 16%, and CAT was decreased by 46%. TRAP was lower (27%) in the myocardium of hypertensive rats than in that of controls. These data suggest that LNA-induced hypertension is associated with increased myocardial oxidative stress.

引用

页码：43 / 50

页数：7

共 164 条

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