Tapetum-specific expression of theOsg6B promoter-β-glucuronidase gene in transgenic rice

被引:35
作者
S. Yokoi
T. Tsuchiya
K. Toriyama
K. Hinata
机构
[1] Tohoku University,Laboratory of Plant Breeding, Faculty of Agriculture
关键词
Tapetum-specific promoter; Transformation; Rice;
D O I
10.1007/BF01146774
中图分类号
学科分类号
摘要
The promoter of an anther tapetum-specific gene,Osg6B, was fused to aβ-glucuronidase (GUS) gene and introduced into rice byAgrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.
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页码:363 / 367
页数:4
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