Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol

被引：99

作者：

Nieves R.A. ^{[1
]}

Ehrman C.I. ^{[1
]}

Adney W.S. ^{[1
]}

Elander R.T. ^{[1
]}

Himmel M.E. ^{[1
]}

机构：

[1] Biotech. Ctr. for Fuels and Chem., National Renewable Energy Laboratory, Golden

来源：

World Journal of Microbiology and Biotechnology | 1997年 / 14卷 / 2期

关键词：

Cellobiase; Cellulase; Industrial enzyme preparations; β-glucosidase;

D O I：

10.1023/A:1008871205580

中图分类号：

学科分类号：

摘要：

Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-D-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-D-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.

引用

页码：301 / 304

页数：3

共 6 条

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