Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase

被引：120

作者：

Ellis D.R. ^{[1
,2
]}

Sors T.G. ^{[1
]}

Brunk D.G. ^{[1
]}

Albrecht C. ^{[1
]}

Orser C. ^{[3
]}

Lahner B. ^{[1
]}

Wood K.V. ^{[4
]}

Harris H.H. ^{[5
,6
]}

Pickering I.J. ^{[5
,7
]}

Salt D.E. ^{[1
]}

机构：

[1] Ctr. Plant Environ. Stress Physiol., 1165 Horticulture Building, Purdue University, West Lafayette

[2] NuCycle Therapy, Inc., Hillside

[3] Arete Associates, Gaithersburg

[4] Chemistry Department, Purdue University, West Lafayette

[5] Stanford Synchrt. Radiat. Laboratory, Stanford Linear Accelerator Center, Menlo Park

[6] School of Chemistry, University of Sydney, Sydney

[7] Department of Geological Science, University of Saskatchewan, Saskatoon

来源：

BMC Plant Biology | / 4卷 / 1期

关键词：

Selenite; SeMet; Selenocysteine; HFBA; Selenate Reduction;

D O I：

10.1186/1471-2229-4-1

中图分类号：

学科分类号：

摘要：

Background: It has become increasingly evident that dietary Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Different forms of Se vary in their chemopreventative efficacy, with Se-methylselenocysteine being one of the most potent. Interestingly, the Se accumulating plant Astragalus bisulcatus (Two-grooved poison vetch) contains up to 0.6% of its shoot dry weight as Se-methylselenocysteine. The ability of this Se accumulator to biosynthesize Se-methylselenocysteine provides a critical metabolic shunt that prevents selenocysteine and selenomethionine from entering the protein biosynthetic machinery. Such a metabolic shunt has been proposed to be vital for Se tolerance in A. bisulcatus. Utilization of this mechanism in other plants may provide a possible avenue for the genetic engineering of Se tolerance in plants ideally suited for the phytoremediation of Se contaminated land. Here, we describe the overexpression of a selenocysteine methyltransferase from A. bisulcatus to engineer Se-methylselenocysteine metabolism in the Se non-accumulator Arabidopsis thaliana (Thale cress). Results: By over producing the A. bisulcatus enzyme selenocysteine methyltransferase in A. thaliana, we have introduced a novel biosynthetic ability that allows the non-accumulator to accumulate Se-methylselenocysteine and γ-glutamylmethylselenocysteine in shoots. The biosynthesis of Se-methylselenocysteine in A. thaliana also confers significantly increased selenite tolerance and foliar Se accumulation. Conclusion: These results demonstrate the feasibility of developing transgenic plant-based production of Se-methylselenocysteine, as well as bioengineering selenite resistance in plants. Selenite resistance is the first step in engineering plants that are resistant to selenate, the predominant form of Se in the environment. © 2004 Ellis et al; licensee BioMed Central Ltd.

引用

页数：11

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