Comparative study of tube assembly in three-dimensional collagen matrix and on Matrigel coats

被引：42

作者：

Montañez E. ^{[1
]}

Casaroli-Marano R.P. ^{[2
]}

Vilaró S. ^{[2
]}

Pagan R. ^{[1
,3
]}

机构：

[1] Advancell, Barcelona Scientific Park, Barcelona

[2] Department of Cell Biology, University of Barcelona, Barcelona

[3] Advancell, Barcelona Scientific Park, E-08028 Barcelona

来源：

Angiogenesis | 2002年 / 5卷 / 3期

关键词：

Angiogenesis; Collagen; HUVEC; In vitro; Matrigel;

D O I：

10.1023/A:1023837821062

中图分类号：

学科分类号：

摘要：

This study compares phenotypic changes of human umbilical endothelial vein cells cultured in three-dimensional collagen matrixes in the presence of basic fibroblast growth factor or on Matrigel coats. Under both conditions, endothelial cells rapidly assembled into an irregular network of tubular structures with a high frequency of intercellular or lumen-like spaces. Tubular structures were characterized and compared by phase-contrast, confocal and electron microscopy. The dominant mechanism of lumen-like formation was highly model-dependent. Ultrastructural analyses of capillary-like structures and the mechanism of lumen-like formation indicated that the in vivo angiogenesis was better reproduced in the collagen model.

引用

页码：167 / 172

页数：5

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