Introduction of a plant intron into the luciferase gene ofPhotinus pyralis

被引：28

作者：

S. Luke Mankin

George C. Allen

William F. Thompson

机构：

[1] North Carolina State University,Department of Botany

[2] North Carolina State University,Department of Genetics

来源：

Plant Molecular Biology Reporter | 1997年 / 15卷 / 2期

关键词：

intron; luciferase;

D O I：

10.1007/BF02812270

中图分类号：

学科分类号：

摘要：

We describe a new luciferase reporter gene,lucINT, for early detection of luciferase activity inAgrobacterium transformation studies, and present improved techniques for the extraction of luciferase that decrease the time needed to quantitate luciferase activity. ThelucINT reporter gene combines the PIV2 intron fromGUSINT withluc*, the modified luciferase gene.lucINTis expressed in plant cells but not inAgrobacterium, allowing earlier detection of gene expression in the presence ofAgrobacterium during transformations in tobacco leaf discs. Stable expression levels oflucINT andluc* in tobacco suspension cultures are compared for two different promoters.

引用

页码：186 / 196

页数：10

共 86 条

[1]

An G.(1985)High efficiency transformation of cultured tobacco cells Plant Physiol. 79 568-570

[2]

Barghchi M.(1995)High-frequency and efficient Meth. Mol. Biol. 44 135-147

[3]

Becker D.(1992)-mediated transformation of Plant Mol. Biol. 20 1195-1197

[4]

Kemper E.(1994) ecotypes “C24” and “Landsberg erecta” using Gene 141 75-77

[5]

Schell J.(1980)New plant binary vectors with selectable markers located proximal to the left T-DNA border Proc. Natl. Acad. Sci. U.S.A. 77 7347-7351

[6]

Masterson R.(1986) luciferase-vectors that contain a modified Mol. Gen. Genet. 205 14-22

[7]

Bonin A.L.(1992) coding sequence allowing convenient transfer into other systems Anal. Biochem. 204 283-291

[8]

Gossen M.(1991)Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Oxford Survey Plant Mol. Cell Biol. 7 255-296

[9]

Bujard H.(1985)Isolation and characterization of a light-inducible, organ-specific gene from potato ( Science 227 1229-1231

[10]

Ditta G.(1987)) and analysis of its expression after tagging and transfer into tobacco and potato shoots EMBO J. 6 3901-7

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