Analysis of genetic relationship in 12 species ofSection Strobus with ISSR markers

Genetic relationship of 12 species ofSection Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%.P. pumila had the highest levels of genetic differentiation andP. flexilis had lowest. Total genetic diversity (HT) of 12 species inSection Strobus was 26.21%, of which intraspecific genetic diversity (Hs) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group includedP. griffithii, P. armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola andP. wangii. The second group includedP. albicaulis, P. pumila, P. flexilis, P. sibirica andP. koraiensis.