The expression of CD40-CD40L and activities of matrix metalloproteinases in atherosclerotic rats

被引：13

作者：

Min Wu

Yu-Guang Li

机构：

[1] Shantou University,Department of Cardiology, First Affiliated Hospital of the Medical College

来源：

Molecular and Cellular Biochemistry | 2006年 / 282卷

关键词：

adhesion factor; atherosclerosis; CD40; CD40L; matrix metalloproteinase;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This study investigated the expression of CD40, CD40 ligand (CD40L) and matrix metalloproteinases (MMPs) in dietary-induced atherosclerosis in rats. Wister rats were fed with high cholesterol diet (As group, n = 6) or with normal diet (N group, n = 6). Blood cells that express CD40 and CD40L were sorted by flow cytometry, the MMP-2 and MMP-9 were measured by zymography method. The morphological locations of MMP-2 and MMP-9 in the aorta were studied with immunohistochemistry and by microscopy. The results showed that the expression of CD40, CD40L and matrix metalloproteinase were higher in As group than those in control group. The MMP-2 and MMP-9 were positive in As group but negative in control group by immunohistochemistry study. Our results suggest that the expression of CD40 and CD40L in the blood cells and the activities of MMP-2 and MMP-9 in plasma were higher in As group than those in Normal group, indicating that they may contribute to the formation of atherosclerosis.

引用

页码：141 / 146

页数：5

共 140 条

[1]

Spronk HM(2004)Blood coagulation and the risk of atherothrombosis: A complex relationship Thromb J 2 12-12

[2]

van der Voort D(2005)Circulating levels of proinflammatory cytokines and neutrophil–platelet aggregates in patients with coronary artery disease Am J Cardiol 95 452-456

[3]

Ten Cate H(2005)Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha J Vasc Surg 41 321-331

[4]

Nijm J(2002)Association between behavior-dependent cardiovascular risk factors and asymptomatic carotid atherosclerosis in a general population Stroke 33 2929-2935

[5]

Wikby A(2005)Atherosclerosis as inflammation Ann Vasc Surg 19 130-138

[6]

Tompa A(2004)The expression of matrix metalloproteinases-9, transforming growth factor-beta1 and transforming growth factor-beta receptor I in human atherosclerotic plaque and their relationship with plaque stability Chin Med J (Engl) 117 1825-1829

[7]

Olsson AG(1996)Requirement for CD40 ligand in costimulation induction, T cell activation, and experimental allergic encephalomyelitis Science 273 1864-1867

[8]

Jonasson L(2004)Regulation of matrix biology by matrix metalloproteinases Curr Opin Cell Biol 16 558-564

[9]

Dardik A(2004)Growth hormone replacement decreases plasma levels of matrix metalloproteinases (2 and 9) and vascular endothelial growth factor in growth hormone-deficient individuals Circulation 109 2405-2410

[10]

Yamashita A(1997)Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40-CD40 ligand signaling in atherosclerosis Proc Natl Acad Sci USA 94 1931-1936

← 1 2 3 4 5 6 7 8 9 10 →