Long term in-vivo studies of a photo-oxidized bovine osteochondral transplant in sheep

被引：18

作者：

Akens M.K. ^{[1
]}

Von Rechenberg B. ^{[1
]}

Bittmann P. ^{[2
]}

Nadler D. ^{[2
]}

Zlinszky K. ^{[1
]}

Auer J.A. ^{[1
]}

机构：

[1] Musculoskeletal Research Unit, Department of Veterinary Surgery, University of Zurich

[2] Sulzer Orthopedics Ltd.

来源：

BMC Musculoskeletal Disorders | / 2卷 / 1期

关键词：

Articular Cartilage; Subchondral Bone; Femoral Condyle; Graft Cartilage; Methylene Blue Solution;

D O I：

10.1186/1471-2474-2-9

中图分类号：

学科分类号：

摘要：

Background: Articular cartilage has limited capacity to repair. Defects greater than 3 mm heal with formation of inferior fibrous cartilage. Therefore, many attempts have been made to find the ideal graft for larger cartilage lesions. Different grafts, such as untreated or cryopreserved osteochondral transplants, have been used with variable success. Methods: Photo-oxidized osteochondral grafts were implanted in both femoral condyles of one ovine knee. Untreated xenogeneic and autogeneic grafts served as controls. Three groups of 8 sheep each were formed and they were sacrificed 6, 12 or 18 months after surgery. Results: The macroscopic evaluation of the condyle and graft showed a well-maintained cartilage surface in most grafts at all time points. However, the host cartilage matrix deteriorated considerably in all xenogeneic, most autogeneic and fewer of the photo-oxidized grafts at 12 and 18 months, respectively. The blue colour of the photo-oxidized grafts resulting from the process of photo-oxidation was visible in all grafts at 6 months, had diminished at 12 months and had completely disappeared at 18 months after surgery. Histologically a loss of matrix staining was almost never noticed in untreated xenografts, transiently at 6 months in photo-oxidized grafts and increased at 12 and 18 months. Fusion between graft and host cartilage could be seen in photo-oxidized grafts at 12 and 18 months, but was never seen in autografts and xenografts. Conclusions: The photo-oxidation of osteochondral grafts and its use as transplant appears to have a beneficial effect on cartilage and bone remodelling. Osteochondral grafts pre-treated with photo-oxidation may be considered for articular cartilage replacement and therefore may delay artificial joint replacements in human patients.

引用

页码：1 / 12

页数：11

共 43 条

[1]

Mankin H.J., Biology of autologous cartilage repair, Steochondral Allografts, pp. 59-72, (1983)

[2]

Caplan A.I., Elyaderani M., Mochizuki Y., Wakitani S., Goldberg V.M., Principles of cartilage repair and regeneration, Clin. Orthop., pp. 254-269, (1997)

[3]

Hunziker E.B., Rosenberg L., Articular cartilage repair, Arthritis and Allied Conditions, 2, pp. 2027-2938, (1997)

[4]

Hurtig M.B., Fretz P.B., Doige C.E., Schnurr D.L., Effects of lesion size and location on equine articular cartilage repair, Can. J. Vet. Res., 52, pp. 137-146, (1988)

[5]

Hanie E.A., Sullins K.E., Powers B.E., Nelson P.R., Healing of full-thickness cartilage compared with full-thickness cartilage and subchondral bone defects in the equine third carpal bone, Equine Vet. J., 24, pp. 382-386, (1992)

[6]

Convery F.R., Akeson W.H., Keown G.H., The repair of large osteochondral defects. An experimental study in horses, Clin. Orthop. Relat. R, 82, pp. 253-262, (1972)

[7]

Weisbrode S.E., Doige C.E., Bone and joints, Special Veterinary Pathology, pp. 499-521, (2001)

[8]

Mow V.C., Ratcliffe A., Rosenwasser M.P., Buckwalter J.A., Experimental studies on repair of large osteochondral defects at a high weight bearing area of the knee joint: A tissue engineering study, J. Biomech. Eng., 113, pp. 198-207, (1991)

[9]

Shahgaldi B.F., Amis A.A., Heatley F.W., McDowell J., Bentley G., Repair of cartilage lesions using biological implants. A comparative histological and biomechanical study in goats, J. Bone Joint Surg. Br., 73, pp. 57-64, (1991)

[10]

Brittberg M., Lindahl A., Nilsson A., Ohlsson C., Isaksson O., Peterson L., Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation, N. Engl. J. Med., 331, pp. 889-895, (1994)

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