Ornamental traits modification by rol genes in Osteospermum ecklonis transformed with Agrobacterium tumefaciens

被引：30

作者：

Giovannini A. ^{[1
]}

Zottini M. ^{[2
]}

Morreale G. ^{[1
]}

Spena A. ^{[2
]}

Allavena A. ^{[1
]}

机构：

[1] Ist. Sperim. per la Floricoltura, 18038 Sanremo (IM), C.so degli Inglesi

[2] Università di Verona, Facoltà di Scienze, 37100 Verona, Strada le Grazie

来源：

In Vitro Cellular & Developmental Biology - Plant | 1999年 / 35卷 / 1期

关键词：

Dimorphotheca; Field trials; Flowering attitude; Growth habit; Transgene segregation;

D O I：

10.1007/s11627-999-0012-2

中图分类号：

学科分类号：

摘要：

Transgenic plants of Osteospermum ecklonis were produced by cocultivation of leaf fragments with Agrobacterium tumefaciens harboring rol genes from A. rhizogenes. The phenotypic alterations caused by the different transgenes were evaluated in field trials. The genetic manipulation produced transgenic plants characterized by the following features: 1) increased number of flowers (e.g., 35SrolC and rolABC); 2) early flowering (e.g., 35SrolC); 3) change of plant growth habit; erect (rolAB, rolABC and 35SrolC) with an increased number of branches (e.g., rolABC). The color of leaves was pale green in 35SrolC and dark green in rolAB transgenic plants. In conclusion this work reports: 1) genetic engineering of the ornamental species O. ecklonis, 2) modification of the main ornamental traits of this species by rol genes, and 3) segregation of the transgenes in the progeny.

引用

页码：70 / 75

页数：5

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