Direct microcontact printing of oligonucleotides for biochip applications

被引：47

作者：

Thibault C. ^{[1
]}

Le Berre V. ^{[2
,3
]}

Casimirius S. ^{[1
]}

Trévisiol E. ^{[2
,3
]}

François J. ^{[2
,3
]}

Vieu C. ^{[1
]}

机构：

[1] LAAS-CNRS, 31077 Toulouse Cedex 4

[2] Biochips Platform Genopole Toulouse, UMR-CNRS 5504, INRA 792, 31077 Toulouse Cedex 4

[3] Laboratoire de Biotechnologie and Bioprocédés, UMR-CNRS 5504, INRA 792, 31077 Toulouse Cedex 4

来源：

Journal of Nanobiotechnology | / 3卷 / 1期

关键词：

Biochips; Detection of mutations; DNA immobilisation; Elastomeric stamp; Microcontact printing;

D O I：

10.1186/1477-3155-3-7

中图分类号：

学科分类号：

摘要：

Background: A critical step in the fabrication of biochips is the controlled placement of probes molecules on solid surfaces. This is currently performed by sequential deposition of probes on a target surface with split or solid pins. In this article, we present a cost-effective procedure namely microcontact printing using stamps, for a parallel deposition of probes applicable for manufacturing biochips. Results: Contrary to a previous work, we showed that the stamps tailored with an elastomeric poly(dimethylsiloxane) material did not require any surface modification to be able to adsorb oligonucleotides or PCR products. The adsorbed DNA molecules are subsequently printed efficiently on a target surface with high sub-micron resolution. Secondly, we showed that successive stamping is characterized by an exponential decay of the amount of transferred DNA molecules to the surface up the 4th print, then followed by a second regime of transfer that was dependent on the contact time and which resulted in reduced quality of the features. Thus, while consecutive stamping was possible, this procedure turned out to be less reproducible and more time consuming than simply re-inking the stamps between each print. Thirdly, we showed that the hybridization signals on arrays made by microcontact printing were 5 to 10-times higher than those made by conventional spotting methods. Finally, we demonstrated the validity of this microcontact printing method in manufacturing oligonucleotides arrays for mutations recognition in a yeast gene. Conclusion: The microcontact printing can be considered as a new potential technology platform to pattern DNA microarrays that may have significant advantages over the conventional spotting technologies as it is easy to implement, it uses low cost material to make the stamp, and the arrays made by this technology are 10-times more sensitive in term of hybridization signals than those manufactured by conventional spotting technology. © 2005 Thibault et al; licensee BioMed Central Ltd.

引用

页数：12

共 13 条

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