A routine method for the determination of retronecine

被引：11

作者：

Jeannette T. ^{[1
]}

Hovermale A. ^{[1
]}

Craig M. ^{[2
]}

Morrie Craig A. ^{[2
]}

机构：

[1] Department of Chemistry, Oregon State University, Corvallis

[2] College of Veterinary Medicine, Oregon State University, Corvallis

来源：

Fresenius' Journal of Analytical Chemistry | 1998年 / 361卷 / 2期

关键词：

Chromatography; Detection Limit; Growth Medium; Bacterial Growth; Area Ratio;

D O I：

10.1007/s002160050862

中图分类号：

学科分类号：

摘要：

A method for the routine determination of the necine base retronecine from biological matrices is described, using gas chromatography for quantification. The biological matrices studied included blended sheep whole rumen fluid and bacterial growth media. The structurally similar compound 2,6-dimethoxypyridine was utilized as an internal standard. Prior to gas chromatography, the bis(heptafluorobutyrate) derivatives of both compounds were formed. The relative percent recoveries of retronecine and the internal standard were 73% and 82%, respectively. The detection limit of retronecine in blended whole rumen fluid was found to be 0.09 μg/mL, and 0.02 μg/mL in bacterial growth media. The precision of the peak area ratio (retronecine to internal standard) was 10% from blended whole rumen fluid, and 14% from bacterial growth media. This method was used to analyze samples from viable cultures incubated with retronecine. © Springer-Verlag 1998.

引用

页码：201 / 206

页数：5

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