Inhibition of human angiogenesis with heparin and hydrocortisone

被引：35

作者：

Jung S.P. ^{[1
,2
]}

Siegrist B. ^{[1
]}

Wade M.R. ^{[3
]}

Anthony C.T. ^{[1
]}

Woltering E.A. ^{[1
,4
,5
,6
]}

机构：

[1] Departments of Surgery, Louisiana State University, Health Sciences Center, New Orleans, LA

[2] Department of Surgery, Soonchunhyang University, Seoul

[3] Public Health and Biostatistics, New Orleans, LA

[4] Stanley S. Scott Cancer Center, New Orleans, LA

[5] Neurosciences Center of Excellence, New Orleans, LA

[6] Veterans Administration Medical Center, New Orleans, LA

来源：

Angiogenesis | 2001年 / 4卷 / 3期

关键词：

Angiogenesis; Endothelium; Heparin; Human; Steroids;

D O I：

10.1023/A:1014089706107

中图分类号：

学科分类号：

摘要：

Angiogenesis is a critical determinant of tumor growth and the development of metastases. Heparin, steroids, and heparin/steroid combinations have been used in a variety of in vitro models and in vivo in animal models as effective inhibitors of angiogenesis. We tested heparin, steroid and heparin/steroid combinations at a variety of concentrations to determine their effect on the human 'angiogenic switch' from a resting to a proliferative endothelium in vessels from three placentas (initiation), and the effect of these compounds on the subsequent growth of a human angiogenic response (promotion). Using full-thickness human placental vein discs cultured in three-dimensional fibrin-thrombin clots, we demonstrated that heparin (300, 3000 μg/ml), steroid (350, 3500 μg/ml), and combinations of heparin/steroid at these doses effectively blocked both initiation and promotion of a human angiogenic response in a dose-dependent fashion. We also demonstrated that high-dose steroid or heparin/steroid treatment for 15 days resulted in disruption of vessel integrity, while treatment with heparin alone produced a suppressed growth rate but had intact vessel architecture. High-dose heparin/steroid treatment could also disrupt a developed angiogenic response and retard further development of an angiogenic response following the cessation of treatment.

引用

页码：175 / 185

页数：10

共 20 条

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