Production of volatile compounds by cheese-ripening yeasts: Requirement for a methanethiol donor for S-methyl thioacetate synthesis by Kluyveromyces lactis

被引：76

作者：

Arfi K. ^{[1
]}

Spinnler H. ^{[2
]}

Tache R. ^{[2
]}

Bonnarme P. ^{[1
]}

机构：

[1] Institut National de la Recherche Agronomique, Laboratoire de Génie et Microbiologie des Procédés Alimentaires

[2] Institut National Agronomique Paris-Grignon, Centre de Biotechnologies Agro-Industrielles

来源：

Applied Microbiology and Biotechnology | 2002年 / 58卷 / 4期

关键词：

Lactis; Kluyveromyces; Thioester; Yarrowia; Yarrowia Lipolytica;

D O I：

10.1007/s00253-001-0925-0

中图分类号：

学科分类号：

摘要：

Five cheese-ripening yeasts (Geotrichum candidum, Saccharomyces cerevisiae, Kluyveromyces lactis, Yarrowia lipolytica and Debaryomyces hansenii) were compared with respect to their ability to generate volatile aroma compounds. K. lactis produced a variety of esters - ethylacetate (EA) being the major one - and relatively limited amounts of volatile sulphur compounds (VSCs). Conversely, G. candidum produced significant amounts of VSCs [with the thioester S-methyl thioacetate (MTA) being the most prevalent] and lower quantities of non-sulphur volatile compounds than K. lactis. We suspect that K. lactis is able to produce and/or accumulate acetyl CoA - a common precursor of MTA and EA - but that it produces limited amounts of methanethiol (MTL); both acetyl CoA and MTL are precursors for MTA synthesis. When supplemented with exogenous MTL, MTA production greatly increased in K. lactiscultures whereas it was unchanged in G. candidum cultures, suggesting that MTL is a limiting factor for MTA synthesis in K. lactis but not in G. candidum. Our results are discussed with respect to L-methionine catabolism.

引用

页码：503 / 510

页数：7

共 37 条

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