A simple method for a mini-preparation of fungal DNA

被引：112

作者：

Saitoh K.-I. ^{[1
]}

Togashi K. ^{[1
]}

Arie T. ^{[1
]}

Teraoka T. ^{[1
,2
]}

机构：

[1] Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo

[2] Laboratory of Plant Pathology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509

来源：

Journal of General Plant Pathology | 2006年 / 72卷 / 6期

关键词：

Ascomycetes; Basidiomycetes; Fungal DNA extraction; Oomycetes; Zygomycetes;

D O I：

10.1007/s10327-006-0300-1

中图分类号：

学科分类号：

摘要：

A simple method was established to prepare DNA from fungal mycelia cultured on an agar plate. The fungi tested successfully with this method contained Zygomycetes, Ascomycetes, Basidiomycetes, and Oomycetes. This method did not require any time-consuming steps to crush or digest mycelia or fractionation in a phenol-chloroform mixture. The DNA was easily extracted by immersing and dispersing the mycelial plugs in a specific buffer (200 mM Tris-HCl, 50 mM ethylenediaminetetraacetic acid, 200 mM NaCl, 1% n-lauroylsarcosine, pH 8.0), then concentrated by ethanol precipitation. The total time to complete the whole procedure was less than 1 h. The quality and quantity were sufficient for polymerase chain reaction amplification and Southern blot analysis. © 2006 The Phytopathological Society of Japan and Springer-Verlag Tokyo.

引用

页码：348 / 350

页数：2

共 13 条

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