Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance

被引：62

作者：

Thi Loc N. ^{[1
]}

Tinjuangjun P. ^{[2
]}

Gatehouse A.M.R. ^{[3
]}

Christou P. ^{[2
]}

Gatehouse J.A. ^{[1
]}

机构：

[1] Department of Biological Sciences, University of Durham, Crop Protection Group

[2] Molecular Biotechnology Unit, John Innes Centre, Norwich NR4 7UH, Colney Lane

[3] Department of Agricultural and Environmental Science, University of Newcastle, Crop Protection Group

来源：

Molecular Breeding | 2002年 / 9卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Clean" DNA; Biolistic transformation; Crop pests; Oryza sativa;

D O I：

10.1023/A:1020333210563

中图分类号：

学科分类号：

摘要：

Biolistic transformation was used to introduce genes encoding the insecticidal proteins snowdrop lectin (Galanthus nivalis agglutinin; GNA) and cry 1Ac Bt toxin (δ-endotoxin from Bacillus thuringiensis) into elite rice (Oryza sativa) cultivars. Plant transformation was carried out in parallel experiments simultaneously by using either whole plasmids containing suitable gene constructs, or the corresponding minimal gene cassettes, which were linear DNA fragments lacking vector sequences excised from the plasmids. Both transformation methods generated similar numbers of independent transformation events. Selected R0 clonal plant lines were further characterised for presence and expression of transgenes. Co-transformation of the unselected genes (cry1Ac and gna) with the selectable marker (hpt) was at least as efficient for transformation with minimal gene cassettes as with whole plasmid DNA, and higher levels of accumulation of the insecticidal gene products GNA and cry1Ac were observed in plants resulting from minimal gene cassette transformation. Insect bioassays with major pests of rice showed that transgenic plants expressing gna showed enhanced resistance to brown planthopper (Nilaparvata lugens), and plants expressing cry1Ac were protected against attack by striped stem borer (Chilo suppressalis). Expression of both transgenes gave protection against both pests, but did not increase protection against either pest significantly over the levels observed in plants containing a single insecticidal transgene.

引用

页码：231 / 244

页数：13

共 40 条

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