Expression of organomercurial lyase in eastern cottonwood enhances organomercury resistance

被引：17

作者：

Che D. ^{[1
]}

Meagher R.B. ^{[2
]}

Rugh C.L. ^{[3
]}

Kim T. ^{[2
]}

Heaton A.C.P. ^{[2
]}

Merkle S.A. ^{[1
]}

机构：

[1] Daniel B. Warnell School of Forest Resources, University of Georgia, Athens

[2] Genetics Department, University of Georgia, Athens

[3] Department of Crop and Soil Sciences, Michigan State University, East Lansing

来源：

In Vitro Cellular & Developmental Biology - Plant | 2006年 / 42卷 / 3期

关键词：

Adventitious shoots; Methylmercury; Phytoremediation; Populus deltoides; Transgenic plants;

D O I：

10.1079/IVP2006771

中图分类号：

学科分类号：

摘要：

Release of inorganic mercury pollutants into shallow aquatic environments has resulted in the bacterial production of a more toxic organic mercury species, methylmercury. The bacterial organomercurial lyase (MerB) catalyses the protonolysis of the carbon-mercury bond and releases Hg(II), a less toxic, non-biomagnified form of mercury. Our objective was to engineer eastern cottonwood (Populus deltoides), a fast-growing tree adapted to growth in riparian environments, with the merB gene to explore its potential for phytoremediation of mercury. We produced multiple eastern cottonwood clones expressing a modified bacterial merB gene, confirmed that the gene was expressed in the transclones and tested the regenerated plants for their ability to tolerate exposure to an organic mercury source, phenylmercuric acetate (PMA), in vitro and in hydroponic culture, compared to wild-type control trees. Transgenic merB plants expressed high levels of MerB protein and showed some evidence of higher resistance to the organic mercury than wild-type plants, producing longer roots under exposure to PMA in vitro, although hydroponic culture results were inconclusive. Our results indicate that in order for merB to be useful in eastern cottonwood trees designed to degrade methylmercury at mercury-contaminated aquatic sites, it will probably need to be combined with other genes such as merA. © 2006 Society for In Vitro Biology.

引用

页码：228 / 234

页数：6

共 35 条

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