Collagen type 1 retards tube formation by human microvascular endothelial cells in a fibrin matrix

被引：43

作者：

Kroon M.E. ^{[1
]}

Van Schiel M.L.J. ^{[1
]}

Van Der Vecht B.v. ^{[1
]}

Van Hinsbergh V.W.M. ^{[1
,2
]}

Koolwijk P. ^{[1
,3
]}

机构：

[1] Gaubius Laboratory TNO-PG, Leiden

[2] Inst. for Cardiovascular Research, Vrije Universiteit, Amsterdam

[3] Gaubius Laboratory TNO-PG, 2301 CE Leiden

来源：

Angiogenesis | 2002年 / 5卷 / 4期

关键词：

Angiogenesis; Collagen type 1; Endothelial cell; Fibrin; Matrix; Matrix metalloproteinases; neovascularisation; Urokinase;

D O I：

10.1023/A:1024540701634

中图分类号：

学科分类号：

摘要：

Angiogenesis, or the formation of new microvessels, is often encountered in pathological situations. A fibrinous exudate can often act as a temporary matrix for the ingrowth of these new microvessels. This matrix consists mainly of fibrin, but is mingled with other plasma components and interstitial collagen fibres. In vitro, capillary-like tube formation can be mimicked by exposing human microvascular endothelial cells (hMVECs), seeded on top of a three-dimensional fibrin matrix, to an angiogenic growth factor (e.g. fibroblast growth factor (FGF)-2) and the cytokine tumour necrosis factor (TNF)-α. Plasmin activity is required in this process. We investigated whether the angiogenic potential of hMVECs was altered by the presence of collagen. The addition of type I collagen to fibrin matrices dose-dependently inhibited tube-formation. Tube-formation in these fibrin/collagen matrices by hMVECs required matrix metalloprotease (MMP) activity, as well as plasmin activity. On a pure collagen type I matrix, hMVECs were not able to form tube-like structures in the matrix but formed sprouts. This sprouting required MMP activity and was, in contrast to the tube-like structures in a fibrin matrix, not influenced by hypoxia. These data indicate that the interaction between endothelial cells and different matrix components is of importance for the angiogenic potential of these cells.

引用

页码：257 / 265

页数：8

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