Construction of a stable microbial community with high cellulose-degradation ability

被引:3
作者
S. Haruta
Z. Cui
Z. Huang
M. Li
M. Ishii
Y. Igarashi
机构
[1] Department of Biotechnology,
[2] University of Tokyo,undefined
[3] Yayoi 1-1-1,undefined
[4] Bunkyo-ku,undefined
[5] Tokyo 113-8657,undefined
[6] Japan,undefined
[7] Department of Agronomy,undefined
[8] China Agricultural University,undefined
[9] Yuanmingyuan West Road,undefined
[10] Haidian District,undefined
[11] Beijing 100094,undefined
[12] P.R. China,undefined
[13] Institute of Microbiology,undefined
[14] Chinese Academy of Sciences,undefined
[15] Beijing 100080,undefined
[16] P.R. China,undefined
来源
Applied Microbiology and Biotechnology | 2002年 / 59卷
关键词
Electrophoresis; Microbial Community; Community Structure; Straw; Rice Straw;
D O I
暂无
中图分类号
学科分类号
摘要
We bred a microbial community capable of degrading rice straw with high efficiency. The microbial community degraded more than 60% of rice straw within 4 days at 50 °C. The high stability of the community's degradation ability was demonstrated by its tolerance of being subcultured several times in medium with/without cellulosic material, being heated to 95 °C, and freezing at –80 °C. The community degraded both nonsterilized and sterilized substrate; and its degradation ability was not affected by pH changes in the medium (initial pH 5–9). PCR-denaturing gradient gel electrophoresis (DGGE) analyses based on 16S rDNA fragments showed that the community structure remained constant after multiple subcultures extending over 2 years. DNA sequence analyses of DGGE bands indicated the coexistence of both aerobic and anaerobic bacteria in the community.
引用
收藏
页码:529 / 534
页数:5
相关论文
empty
未找到相关数据