Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: Manipulation of plant growth regulators and sucrose

被引：27

作者：

Xu J.F. ^{[1
,3
]}

Ying P.Q. ^{[2
]}

Han A.M. ^{[2
]}

Su Z.G. ^{[1
]}

机构：

[1] State Key Lab. of Biochem. Eng., Institute of Chemical Metallurgy, Chinese Academy of Science, Beijing

[2] Institute of Biochemical Engineering, Dalian University of Technology, Dalian

[3] Department of Chemical Engineering, Ohio University, Athens

来源：

Plant Cell, Tissue and Organ Culture | 1998年 / 55卷 / 1期

基金：

中国国家自然科学基金;

关键词：

Chinese herb differentiation; Secondary metabolites;

D O I：

10.1023/A:1026489515174

中图分类号：

学科分类号：

摘要：

Liquid-cultivated compact callus aggregates (CCA) of Rhodiola sachalinensis were initiated from the explant of stems. Microscopic analysis showed that CCA displayed some level of cellular/tissue differentiation, which was perhaps responsible for the increased salidroside production. A higher ratio of cytokinin/auxin was found to be beneficial both for CCA growth and salidroside accumulation. Sucrose (< 100 g l-1) had a positive effect on salidroside synthesis, which was not due to an osmotic effect of the medium. The medium containing 40 g l-1 sucrose, 1.5 μM naphthaleneacetic acid and 15 μM 6-benzylaminopurine yielded ca. 120 mg l-1 salidroside after 24 days of culture.

引用

页码：53 / 58

页数：5

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