Isolation of the 3-phosphoglycerate kinase gene of the arbuscular mycorrhizal fungus glomus mosseae (Nicol. and Gerd.) Gerdemann and Trappe

被引：24

作者：

Harrier L.A. ^{[1
]}

Wright F. ^{[3
]}

Hooker J.E. ^{[2
]}

机构：

[1] Biotechnology Unit, Plant Science Division, Scottish Agricultural College, Edinburgh EH9 3JG, Mains Road

[2] School of Applied Sciences, University of Glamorgan, Pontypriod, Mid-Glamorgan

[3] Biomathematics and Stat. Scotland, Scottish Crop Research Institute, Invergowrie

来源：

Current Genetics | 1998年 / 34卷 / 5期

关键词：

3-Phosphoglycerate kinase; Arbuscular mycorrhizal fungi; Differential RNA display; Glomus mosseae;

D O I：

10.1007/s002940050411

中图分类号：

学科分类号：

摘要：

The 3-phosphoglycerate kinase (PGK) gene of the arbuscular mycorrhizal fungus Glomus mosseae has been isolated by differential RNA display (DD). Experimentally, the technique of DD was utilized to simultaneously compare the mRNA transcript populations from Lycopersicon esculentum root systems colonized by the arbuscular mycorrhizal fungus G. mosseae and non-mycorrhizal plants. A differentially expressed band was isolated and cloned from mycorrhizal tissue. The isolated DD fragment was screened and sequenced. A reverse transcription-PCR (RT-PCR) technique detected transcripts in germinated spores and G. mosseae-colonized root systems but not in uncolonised root systems. The full-length cDNA was isolated by RACE and corresponded to the 3-phosphoglycerate kinase gene of G. mosseae. The cDNA encodes a polypeptide of 416 amino acids, with a predicted molecular weight of 44 764 Da. The PGK proteins shows high homology to those PGKs of other fungi and the phylogenetic relationship among 14 isolated fungal PGK sequences is illustrated.

引用

页码：386 / 392

页数：6

共 48 条

[1] Azevedo J.E., Tropschug M., Werner S., Primary sructure and in vitro expression of the Neurospora crassa phosphoglycerate kinase DNA sequence, J DNA Seq Mapping, 2, pp. 265-267, (1992)
[2] Bruns T.D., Vilgalys R., Barns S.M., Gonzalez D., Hibbett D.S., Lane D.J., Simon L., Stickel S., Szaro T.M., Weisburg D.S., Sogin M.L., Evolutionary relationships within the fungi: Analyses of nuclear small subunit rRNA sequences, Mol Phylog Evol, 1, pp. 231-241, (1993)
[3] Burleigh S.H., Harrison M.J., A cDNA from the arbuscular mycorrhizal fungus Glomus versiforme with homology to a cruciform DNA-binding protein from Ustilago maydis, Mycorrhiza, 7, pp. 307-312, (1998)
[4] Chambers A., Tsang J.S.H., Stanway C., Kingsman A.J., Kingsman S.M., Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAP1, Mol Cell Biol, 9, pp. 5516-5524, (1989)
[5] Clements J.M., Roberts C.F., Molecular cloning of the 3-phosphoglycerate kinase gene from Aspergillus nidulans, Curr Genet, 9, pp. 293-298, (1985)
[6] Clements J.M., Roberts C.F., Transcription and processing signals in the 3-phosphoglycerate kinase gene from Aspergillus nidulans, Gene, 44, pp. 97-105, (1986)
[7] Felsenstein J., An alternative least squares approach to inferring phylogenies from pairwise distances, Systematic Biol, 46, pp. 101-111, (1997)
[8] Fitch W.M., Margoliash E., Construction of phylogenetic trees, Science, 155, pp. 279-284, (1967)
[9] Forbes P.J., Millam S., Hooker J.E., Harrier L.A., Transformation of the arbuscular mycorrhizal fungus Gigaspora rosea Nicolson and Schenck using particle bombardment, Mycol Res, 102, pp. 497-501, (1998)
[10] Fournier A., Fleer R., Yeh P., Mayaux J.F., The primary structure of the 3- Phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis, Nucleic Acids Res, 18, (1990)

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