Highly efficient, 5′-sequence-specific transgene silencing in a complex polyploid

被引：21

作者：

Birch R.G. ^{[1
]}

Bower R.S. ^{[1
]}

Elliott A.R. ^{[1
]}

机构：

[1] Department of Botany/BIOL, The University of Queensland

来源：

Tropical Plant Biology | 2010年 / 3卷 / 2期

基金：

澳大利亚研究理事会;

关键词：

Luciferase; Post-transcriptional silencing; Saccharum; Sugarcane; Ubiquitin promoter;

D O I：

10.1007/s12042-010-9047-0

中图分类号：

学科分类号：

摘要：

Sugarcane, a complex polyploid, shows highly efficient and rapidly imposed silencing of diverse transgene constructs. Silencing is 5′-sequence-specific, copy-number independent, developmentally regulated and post-transcriptional in the plants first regenerated from transgenic callus. The results challenge some early generalizations from studies in model dicotyledonous plants, about causes of transgene silencing and approaches to avoid the problem. We reason that patchy and progressive transgene silencing, seen in most other plant species, may be associated with increasing endopolyploidy during maturation of differentiated tissues. The experimentally tractable sugarcane system allows features likely to trigger or protect from silencing to be tested, and ultimately controlled to improve the efficiency of plant improvement by genetic transformation. © 2010 Springer Science+Business Media, LLC.

引用

页码：88 / 97

页数：9

共 67 条

[1]

Baulcombe D.C., English J.J., Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants, Curr Opin Biotechnol, 7, pp. 173-180, (1996)

[2]

Birch R.G., Plant transformation: Problems and strategies for practical application, Annu Rev Plant Physiol Plant Mol Biol, 48, pp. 297-326, (1997)

[3]

Birch R.G., Bower R., Elliott A.R., Potier B.A.M., Franks T., Cordeiro G., Expression of foreign genes in sugarcane, Proc Int Soc Sugarcane Technol XXII Congress, pp. 368-373, (1996)

[4]

Bird A.P., Gene number, noise reduction and biological complexity, Trends Genet, 11, pp. 94-100, (1995)

[5]

Bower R., Elliott A.R., Potier B.A.M., Birch R.G., High-efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markers, Mol Breed, 2, pp. 239-249, (1996)

[6]

Bradford M., A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein-dye binding, Anal Biochem, 72, pp. 248-254, (1976)

[7]

Brodersen P., Voinnet O., The diversity of RNA silencing pathways in plants, Trends Genet, 22, pp. 268-280, (2006)

[8]

Cesarone C.F., Bolognesi C., Santi L., Improved microfluorometric DNA determination in biological material using 33258 Hoechst, Anal Biochem, 100, pp. 188-197, (1979)

[9]

Chamberlain D.A., Brettel R.I.S., Last D.I., Witrzens B., McElroy D., Dolferus R., Dennis E.S., The use of the Emu promoter with antibiotic and herbicide resistance genes for the selection of transgenic wheat callus and rice plants, Aust J Plant Physiol, 21, pp. 95-112, (1994)

[10]

Chapman E.J., Carrington J.C., Specialization and evolution of endogenous small RNA pathways, Nat Rev Genet, 8, pp. 884-896, (2007)

← 1 2 3 4 5 6 7 →