Cross-reactivity between HLA-A2-restricted FLU-M1:58-66 and HIV p17 GAG:77-85 epitopes in HIV-infected and uninfected individuals

被引：35

作者：

Paula M Acierno

Danforth A Newton

Edwin A Brown

Lou Anne Maes

John E Baatz

Sebastiano Gattoni-Celli

机构：

[1] Department of Radiation Oncology, Medical University of South Carolina, Charleston

[2] Department of Medicine, Medical University of South Carolina, Charleston

[3] Department of Pathology, Medical University of South Carolina, Charleston

[4] Department of Pediatrics, Medical University of South Carolina, Charleston

[5] Dept. of Microbiology/Immunology, Medical University of South Carolina, Charleston

来源：

Journal of Translational Medicine | / 1卷 / 1期

关键词：

Human Immunodeficiency Virus; Influenza; Seronegative Donor;

D O I：

10.1186/1479-5876-1-3

中图分类号：

学科分类号：

摘要：

Background. The matrix protein of the influenza A virus and the matrix and capsid proteins of the human immunodeficiency virus (HIV) share striking structural similarities which may have evolutionary and biological significance. These similarities led us to hypothesize the existence of cross-reactivity between HLA- A2-restricted FLU-M1:58-66 and HIV-1 p17 GAG:77-85 epitopes. Methods. The hypothesis that these two epitopes are cross-reactive was tested by determining the presence and extent of FLU/GAG immune cross-reactivity in lymphocytes from HIV-seropositive and seronegative HLA-A2+ donors by cytotoxicity assays and tetramer analyses. Moreover, the molecular basis for FLU/GAG cross-reactivity in HIV-seropositive and seronegative donors was studied by comparing lymphocyte-derived cDNA sequences corresponding to the TCR-β variable regions, in order to determine whether stimulation of lymphocytes with either peptide results in the expansion of identical T-cell clonotypes. Results. Here, we report evidence of cross-reactivity between FLU-M1:58-66 and HIV-1 p17 GAG:77-85 epitopes following in vitro stimulation of PBMC derived from either HIV-seropositive or seronegative HLA-A2+ donors as determined by cytotoxicity assays, tetramer analyses, and molecular clonotyping. Conclusion. These results suggest that immunity to the matrix protein of the influenza virus may drive a specific immune response to an HLA-A2-restricted HIV gag epitope in HIV-infected and uninfected donors vaccinated against influenza. © 2003 Acierno et al; licensee BioMed Central Ltd.

引用

页数：11

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