Changes of regulatory T cells in Graves' disease.

被引：32

作者：

Wang H.

Zhao S.

Tang X.

Li J.

Zou P.

机构：

来源：

Journal of Huazhong University of Science and Technology | 2006年 / 26卷 / 5期

关键词：

Graves’ disease; regulatory T cell; IL-10; FOXP3;

D O I：

10.1007/s11596-006-0515-6

中图分类号：

学科分类号：

摘要：

The immune mechanism of Graves' diseases (GD) and the roles of regulator T cells were investigated. In 32 patients with GD (GD group) and 20 healthy volunteers (control group), flow cytometry was used to detect the proportion of CD4+CD25+ cells, MACS to isolate CD4+ CD25+ cells, RT-PCR to assay the expression of FOXP3, and ELISA to test the level of IL-10, respectively. It was found that there was no significant change in the proportion of CD4+CD25+ T cells between GD group and control group (P>0.05), while secretion of IL-10 and expression of FOXP3 in GD group were lower than control group (P<0.01 and P<0.05, respectively). In conclusion, though the proportion of regulatory T cells of peripheral blood lymphocytes in the patients with GD, the functions of them were significantly weakened, which might be a pathogenic factor in GD.

引用

页码：545 / 547

页数：2

共 30 条

[1]

Hori S.(2004)Foxp3: a critical regulator of the development and function of regulatory T cells Microbes Infect 6 745-751

[2]

Sakaguchi S.(1990)Use of reverse transcriptase polymerase chain reaction to monitor expression of intronless genes Biotechniques 9 262-562

[3]

Grillo M.(2004)Naturally arising CD4 Annu Rev Immunol 22 531-6327

[4]

Margolis F. L.(2003) regulatory T cells for immunologic self-tolerance and negative control of immune responses J Immunol 171 6323-344

[5]

Sakaguchi S.(2005)The regulatory T cell family: Distinct subsets and their interrelations Nat Immunol 6 338-1248

[6]

Jonuleit H.(2003)Mechanisms of suppression by suppressor T cells J Immunol 170 1240-246

[7]

Schmitt E.(2002)Role for IL-10 in suppression mediated by peptide-induced regulatory T cells J Exp Med 196 237-95

[8]

von Boehmer H.(2000)CD4(+)CD25(+) regulatory T cells can mediate suppressor function in the absence of transforming growth factor beta1 production and responsiveness Pflugers Arch 440 R94-73

[9]

Sundstedt A.(2001)Changes in Th1/Th2 cytokine balance in Graves’ disease Nat Genet 27 68-1061

[10]

O’Neill E. J.(2003)Disruption of a new for khead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse Science 299 1057-336

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