Assessment of a hepatitis C virus antibody assay in saliva for epidemiological studies

被引：36

作者：

Bello P.-Y. ^{[1
,2
]}

Pasquier C. ^{[2
]}

Gourney P. ^{[2
]}

Puel J. ^{[2
]}

Izopet J. ^{[2
]}

机构：

[1] Observ. Reg. la S. en Midi-Pyrenees, Faculté de Médecine, F-31073 Toulouse Cédex

[2] Laboratoire de Virologie, Ctr. Hosp. Universitaire Purpan, F-31059 Toulouse Cédex, Place du Docteur Baylac

来源：

European Journal of Clinical Microbiology and Infectious Diseases | 1998年 / 17卷 / 8期

关键词：

Hepatitis; Internal Medicine; Optical Density; Epidemiological Study; Significant Influence;

D O I：

10.1007/BF01708621

中图分类号：

学科分类号：

摘要：

The performance of a commercially available assay for detection of hepatitis C virus (HCV) antibody in saliva samples was assessed. Samples of saliva were collected from 270 individuals whose HCV antibody status was determined by serum assay (161 HCV-positive, 109 HCV-negative). The saliva samples were tested for the presence of HCV antibodies using a modified protocol. The sensitivity was 94.4% (95% CI, 89.3-97.2%) and the specificity 99.1% (95% CI, 94.3-100%). Although the optical density in tests on HIV-positive individuals was lower than that among HIV-negative individuals, the HIV status had no significant influence on the results of the HCV assay in saliva. These findings suggest that tests on saliva can be useful in epidemiological studies for estimating the prevalence of HCV in populations that are difficult to reach.

引用

页码：570 / 572

页数：2

共 11 条

[1] Conrycantilena C., Hepatitis C virus diagnostics - Technology, clinical applications and impacts, Trends in Biotechnology, 15, pp. 71-76, (1997)
[2] Frerichs R.R., Silarug N., Eskes N., Pagcharoenpol P., Rodklai A., Thangsupachai S., Wongba C., Saliva-based HIV-antibody testing in Thailand, AIDS, 8, pp. 885-894, (1994)
[3] Noone A., Durante A.J., Brady A.R., Majid F., Swan A.V., Parry J.V., Hart G.J., Connell J.A., Perry K.R., Joce R.E., Gill N.O., HIV infection in injecting drug users attending centres in England and Wales, 1990-1991, AIDS, 7, pp. 1501-1507, (1993)
[4] Connell J.A., Parry J.V., Mortimer P.P., Duncan J., Novel assay lor the detection of immunoglobulin G antihuman immunodeficiency virus in untreated saliva and urine, Journal of Medical Virology, 41, pp. 159-164, (1993)
[5] Sherman K.E., Creager R.L., O'Brien J., Sargent S., Piacentini S., Thieme T., The use of oral fluid for hepatitis C antibody screening, American Journal of Gastroenterology, 89, pp. 2025-2027, (1994)
[6] Fleiss J.F., Statistical Methods for Rates and Proportions, 321, (1981)
[7] Thieme T., Yoshihara P., Piacentini S., Beller M., Clinical evaluation of oral fluid samples for diagnosis of viral hepatitis, Journal of Clinical Microbiology, 30, pp. 1076-1079, (1992)
[8] Mcintyre P.G., Laszlo J., Appleyard K., Ogden G.R., Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid, European Journal of Clinical Microbiology & Infectious Diseases, 15, pp. 882-884, (1996)
[9] Emmons W.W., Paparello S.F., Decker C.F., Sheffield J.M., Lowe-Bey F.H., A modified ELISA and Western blot accurately determine anti-human immunodeficiency virus type 1 antibodies in oral fluids obtained with a special collecting device, Journal of Infectious Diseases, 171, pp. 1406-1410, (1995)
[10] Frerichs R.R., Accuracy of saliva test for HIV antibody, Journal of Acquired Immune Deficiency Syndrome and Human Retrovirology, 10, pp. 484-485, (1995)

← 1 2 →